The mitochondrial alternative oxidase Aox1 is needed to cope with respiratory stress but dispensable for pathogenic development in Ustilago maydis
Por:
Cardenas-Monroy, Christian A., Pohlmann, Thomas, Pinon-Zarate, Gabriela, Matus-Ortega, Genaro, Guerra, Guadalupe, Feldbruegge, Michael, Pardo, Juan Pablo
Publicada:
8 mar 2017
Resumen:
The mitochondrial alternative oxidase is an important enzyme that allows
respiratory activity and the functioning of the Krebs cycle upon
disturbance of the respiration chain. It works as a security valve in
transferring excessive electrons to oxygen, thereby preventing potential
damage by the generation of harmful radicals. A clear biological
function, besides the stress response, has so far convincingly only been
shown for plants that use the alternative oxidase to generate heat to
distribute volatiles. In fungi it was described that the alternative
oxidase is needed for pathogenicity. Here, we investigate expression and
function of the alternative oxidase at different stages of the life
cycle of the corn pathogen Ustilago maydis (Aox1). Interestingly,
expression of Aox1 is specifically induced during the stationary phase
suggesting a role at high cell density when nutrients become limiting.
Studying deletion strains as well as overexpressing strains revealed
that Aox1 is dispensable for normal growth, for cell morphology, for
response to temperature stress as well as for filamentous growth and
plant pathogenicity. However, during conditions eliciting respiratory
stress yeast-like growth as well as hyphal growth is strongly affected.
We conclude that Aox1 is dispensable for the normal biology of the
fungus but specifically needed to cope with respiratory stress.
Filiaciones:
Cardenas-Monroy, Christian A.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Mexico City, DF, Mexico
Pohlmann, Thomas:
Heinrich Heine Univ Dusseldorf, Dept Biol, Cluster Excellence Plant Sci, Inst Microbiol, Dusseldorf, Germany
Pinon-Zarate, Gabriela:
Univ Nacl Autonoma Mexico, Fac Med, Dept Biol Celular & Tisular, Mexico City, DF, Mexico
Matus-Ortega, Genaro:
Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Mexico City, DF, Mexico
Guerra, Guadalupe:
Inst Politecn Nacl, Dept Microbiol, Escuela Nacl Ciencias Biol, Mexico City, DF, Mexico
Feldbruegge, Michael:
Heinrich Heine Univ Dusseldorf, Dept Biol, Cluster Excellence Plant Sci, Inst Microbiol, Dusseldorf, Germany
Pardo, Juan Pablo:
Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Mexico City, DF, Mexico
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