Maturation Phenotype of Peripheral Blood Monocyte/Macrophage After Stimulation with Lipopolysaccharides in Irritable Bowel Syndrome
Por:
Rodriguez-Fandino, Oscar A., Hernandez-Ruiz, Joselin, Lopez-Vidal, Yolanda, Charua-Guindic, Luis, Escobedo, Galileo, Schmulson, Max J.
Publicada:
1 abr 2017
Resumen:
Background/Aims
Abnormal immune regulation and increased intestinal permeability
augmenting the passage of bacterial molecules that can activate immune
cells, such as monocytes/macrophages, have been reported in irritable
bowel syndrome (IBS). The aim was to compare the maturation phenotype of
monocytes/macrophages (CD14+) from IBS patients and controls in the
presence or absence of Escherichia coli lipopolysaccharides (LPS), in
vitro.
Methods
Mononuclear cells were isolated from peripheral blood of 20 Rome II-IBS
patients and 19 controls and cultured with or without LPS for 72 hours.
The maturation phenotype was examined by flow cytometry as follows:
M1-Early (CD11c(+)CD206(-)), M2-Advanced (CD11c(-)CD206(+)CX3CR1(+));
expression of membrane markers was reported as mean fluorescence
intensity (MFI). The Mann-Whitney test was used and significance was set
at P < 0.05.
Results
In CD14+ cells, CD11c expression decreased with vs without LPS both in
IBS (MFI: 8766.0 +/- 730.2 vs 12 920.0 +/- 949.2, P < 0.001) and
controls (8233.0 +/- 613.9 vs 13 750.0 +/- 743.3, P < 0.001). M1-Early
cells without LPS, showed lower CD11c expression in IBS than controls
(MFI: 11 540.0 +/- 537.5 vs 13 860.0 +/- 893.7, P = 0.040), while both
groups showed less CD11c in response to LPS (P < 0.01). Furthermore, the
percentage of ``Intermediate'' (CD11c(+)CD206(+)CX3CR1(+)) cells
without LPS, was higher in IBS than controls (IBS = 9.5 +/- 1.5% vs C =
4.9 +/- 1.4%, P < 0.001). Finally, fractalkine receptor (CX3CR1)
expression on M2-Advanced cells was increased when treated with LPS in
controls but not in IBS (P < 0.001).
Conclusions
The initial phase of monocyte/macrophage maturation appears to be more
advanced in IBS compared to controls. However, the decreased CX3CR1 in
patients with IBS, compared to controls, when stimulated with LPS
suggests a state of immune activation in IBS.
Filiaciones:
Rodriguez-Fandino, Oscar A.:
Univ Nacl Autonoma Mexico, Hosp Gen Mexico, Fac Med, Unidad Invest Med Expt,Lab Higado Pancreas & Moti, Dr Balmis 148, Mexico City 06726, DF, Mexico
Fdn Univ Unitrop, Direcc Invest, Yopal, Colombia
Hernandez-Ruiz, Joselin:
Univ Nacl Autonoma Mexico, Hosp Gen Mexico, Fac Med, Unidad Invest Med Expt,Lab Higado Pancreas & Moti, Dr Balmis 148, Mexico City 06726, DF, Mexico
Lopez-Vidal, Yolanda:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Programa Immunol Mol Microbiana, Mexico City, DF, Mexico
Charua-Guindic, Luis:
Hosp Gen Mexico City, Unidad Coloproctol, Mexico City, DF, Mexico
Escobedo, Galileo:
Univ Nacl Autonoma Mexico, Hosp Gen Mexico, Fac Med, Unidad Invest Med Expt,Lab Higado Pancreas & Moti, Dr Balmis 148, Mexico City 06726, DF, Mexico
Schmulson, Max J.:
Univ Nacl Autonoma Mexico, Hosp Gen Mexico, Fac Med, Unidad Invest Med Expt,Lab Higado Pancreas & Moti, Dr Balmis 148, Mexico City 06726, DF, Mexico
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