Piqueria trinervia as a source of metabolites against Giardia intestinalis
Por:
Rufino-Gonzalez, Yadira, Ponce-Macotela, Martha, Jimenez-Estrada, Manuel, Jimenez-Fragoso, Candy N., Palencia, Guadalupe, Sanson-Romero, Gabriel, Anzo-Osorio, Anahi, Martinez-Gordillo, Mario N.
Publicada:
19 may 2017
Resumen:
Context: Piqueria trinervia Cav. (Asteraceae) is a plant species with a
long history in traditional medicine to cure diarrhoea and other
digestive disorders.
Objective: The study investigates the antigiardial activity of piquerol,
trinervinol, red oil and two fractions (F1 and F2) from P. trinervia.
Materials and methods: P. trinervia was collected in the Ajusco in
Mexico City. Aerial parts were ground and mixed with water to obtain the
extract, which was treated with dichloromethane to isolate piquerol and
trinervinol (P & T). Remnants were the red oil, fractions 1 and 2 (RO,
F1 & F2). Trophozoites of Giardia intestinalis were treated with P, T,
RO, F1 and F2 at different concentrations (0.78- 200 mu g/mL) for 48 h.
Antigiardial activity was measured using the methylene blue reduction,
and the cytotoxicity assayed on human fibroblasts and Vero cells by
reduction of tetrazolium salts.
Results: Trinervinol and piquerol showed antigiardial activity with an
IC50 = 2.03 and 2.42 mu g/mL, and IC90 = 13.03 and 8.74 mu g/mL,
respectively. The concentrations of trinervinol (CC50 =590 mu g/mL) and
piquerol (CC50 = 501 mu g/mL) were not cytotoxic to human fibroblasts.
Conclusions: Compounds from P. trinervia showed antigiardial activity;
to enhance this activity, piquerol and trinervinol can be chemically
modified.
Filiaciones:
Rufino-Gonzalez, Yadira:
Inst Nacl Pediat, Lab Parasitol Expt, Mexico City, DF, Mexico
Ponce-Macotela, Martha:
Inst Nacl Pediat, Lab Parasitol Expt, Mexico City, DF, Mexico
Jimenez-Estrada, Manuel:
Univ Nacl Autonoma Mexico, Inst Quim, Mexico City, DF, Mexico
Jimenez-Fragoso, Candy N.:
Inst Nacl Pediat, Lab Parasitol Expt, Mexico City, DF, Mexico
Palencia, Guadalupe:
Inst Nacl Neurol & Neurocirug, Lab Neuroinmunol, Mexico City, DF, Mexico
Sanson-Romero, Gabriel:
Univ Nacl Autonoma Mexico, Inst Quim, Mexico City, DF, Mexico
Anzo-Osorio, Anahi:
Inst Nacl Pediat, Metodol Invest, Mexico City, DF, Mexico
Martinez-Gordillo, Mario N.:
Inst Nacl Pediat, Lab Parasitol Expt, Mexico City, DF, Mexico
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