Inhibitory effect of ethinylestradiol on coagulation factors in rats
Por:
Franco-Murillo, Yanira, Jaimez, Ruth
Publicada:
1 abr 2017
Resumen:
Epidemiological and experimental data have indicated the beneficial and
adverse effects of estrogenic replacement therapy. In the present study,
we explored the effect of ethinylestradiol (EE) and 17 beta-estradiol
(E-2) on screening tests, prothrombin time (PT) and activated partial
thromboplastin time (APTT), as well as the activity of coagulation
factors (FVII, FX, FXI, and FXII) in male Wistar rats. Animals were
injected subcutaneously during three consecutive days with EE or E2 (1,
3, 10, and 30 mg/kg) and propylene glycol (0.3 ml; vehicle, V). EE
produced significant increments (P<0.05) on PT (8, 13, 15, and 10%) and
APTT (32, 35, and 28%), whereas E2 did not show any effect. EE
diminished the activity of factors VII (-10, -13, and -10%) and X (-10,
-9, -15, and -14%; P<0.05), and E2 (1 mg/kg) produced a modest
increment (8%; P<0.05) on FX only. E2 (10 mg/kg) showed a diminution of
9% (P<0.05), while EE did not produce any response on factor XII. EE
diminished (-15, -14, -19, and -17%) but E2 augmented (10, 14, 24, and
24%) factor XI activity (P<0.05). Our findings suggest that EE and E2
produce different effects on coagulation and that EE seems to act across
an inhibitory mechanism of coagulation factor activity in the present
experimental model.
Filiaciones:
Univ Nacl Autonoma Mexico, Fac Med, Dept Farmacol, Ciudad De Mexico 04510, Mexico
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