Sensitivity of the Mitochondrial Unspecific Channel of Saccharomyces cerevisiaeto Butane-1,4-Bisphosphate, a Competitive Inhibitor of Fructose-1,6-Bisphosphate-Aldolase.
Por:
Rosas-Lemus, M., Chiquete-Felix, N., Ruiz-Perez, K., Rigoulet, M., Devin, A., Hernandez-Rodriguez, M., Uribe-Carvajal, S.
Publicada:
16 jul 2016
Categoría:
Chemistry (miscellaneous)
Resumen:
Fructose-1,6-bisphosphate (F1,6BP) promotes closure ofthe S. cerevisiae
mitochondrial unspecific channel (ScMUC) enhancing coupling of oxidative
phosphorylation and probably contributing to the Crabtree effect. In
contrast the closely related glucose-6-phosphate (G6P) opens ScMUC. In
order to understand the putative effects of F1,6BPon the mitochondrial
unspecific channel, butane-1,4-bisphosphate (B1,4BP), a
non-me-tabolizable, competitive inhibitor of F1,6BP-aldolase, was
synthesized and tested on isolated mitochondria. B1,4BP closed ScMUC and
its effects were reverted by increasing concentrations of G6P. Thus, in
regard to its effects on ScMUC, and most likely due to structural
similarities allowing it to bind to the same sites, B1,4BP is an analog
of F1,6BP.
Filiaciones:
Univ Nacl Autonoma Mexico, Dept Mol Genet, Inst Fisiol Celular, Ciudad Univ,Apdo Postal 70-242, Mexico City 70242, DF, Mexico
Inst Biochim & Genet Cellulaires, CNRS UMR 5095, Bordeaux, France
Univ Nacl Autonoma Mexico, Dept Quim Organ, Inst Quim, Circuito Exterior S-N,Ciudad Univ, Mexico City 04510, DF, Mexico
|