Glycine receptor subunits expression in the developing rat retina
Por:
Sánchez-Chávez G., Velázquez-Flores M.Á., Ruiz Esparza-Garrido R., Salceda R.
Publicada:
1 sep 2017
Resumen:
Background and methods: Glycine receptor (GlyR) consists of two alpha
(1-4) and three beta subunits. Considerable evidence indicates that the
adult retina expresses the four types of alpha subunits; however, the
proportion of these subunits in adult and immature retina is almost
unknown. In this report we have studied mRNA and the protein expression
of GlyR subunits in the retina during postnatal rat development by
Real-Time qRT-PCR and western blot.
Results: mRNA and protein expression indicated a gradual increase of the
alpha 1, alpha 3, alpha 4 and beta GlyR subunits during postnatal ages
tested. The mRNA beta subunit showed higher expression levels (similar
to 3 fold) than those observed for the alpha 1 and alpha 3 subunits.
Very interestingly, the alpha 2 GlyR subunit had the highest expression
in the retina, even in the adult.
Conclusions: These results revealed the expression of GlyR at early
postnatal ages, supporting its role in retina development. In addition,
our results indicated that the adult retina expressed a high proportion
of the alpha 2 subunit, suggesting the expression of monomeric and/or
heteromeric receptors. A variety of studies are needed to further
characterize the role of the specific subunits in both adult and
immature retina. (C) 2017 Elsevier Ltd. All rights reserved.
Filiaciones:
División de Neurociencias, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Laboratorio de Genómica Funcional, Unidad de Investigación Médica en Genética Humana del Hospital de Pediatría “Silvestre Frenk Freund”, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, Mexico
Catedrática CONACyT, Laboratorio de Genómica Funcional, Unidad de Investigación Médica en Genética Humana del Hospital de Pediatría “Silvestre Frenk Freund”, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, Mexico
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