Dehydroepiandrosterone increases the number and dendrite maturation of doublecortin cells in the dentate gyrus of middle age male Wistar rats exposed to chronic mild stress
Por:
Herrera-Perez, J. J., Martinez-Mota, L., Jimenez-Rubio, G., Ortiz-Lopez, L., Cabrera-Munoz, E. A., Galindo-Sevilla, N., Zambrano, E., Hernandez-Luis, F., Ramirez-Rodriguez, G. B., Flores-Ramos, M.
Publicada:
15 mar 2017
Categoría:
Behavioral neuroscience
Resumen:
Aging increases the vulnerability to stress and risk of developing
depression. These changes have been related to a reduction of
dehydroepiandrosterone (DHEA) levels, an adrenal steroid with anti
stress effects. Also, adult hippocampal neurogenesis decreases during
aging and its alteration or impaired is related to the development of
depression. Besides, it has been hypothesized that DHEA increases the
formation of new neurons. However, it is unknown whether treatment with
DHEA in aging may stimulate the dendrite maturation of newborn neurons
and reversing depressive-like signs evoked by chronic stress exposure.
Here aged male rats (14 months old) were subjected to a scheme of
chronic mild stress (CMS) during six weeks, received a treatment with
DHEA from the third week of CMS. Changes in body weight and sucrose
preference (SP) were measured once a week. DHEA levels were measured in
serum, identification of doublecortin-(DCX)-, BrdU- and
BrdU/NeuN-labeled cells was done in the dentate gyrus of the
hippocampus. CMS produced a gradual reduction in the body weight, but no
changes in the SP were observed. Treatment enhanced levels of DHEA, but
lack of recovery on body weight of stressed rats. Aging reduced the
number of DCX-, BrdU- and BrdU/NeuN-cells but DHEA just significantly
increased the number of DCX-cells in rats under CMS and controls,
reaching levels of young non-stressed rats (used here as a reference of
an optimal status of health). In rats under CMS, DHEA facilitated
dendritic maturation of immature new neurons. Our results reveal that
DHEA improves neural plasticity even in conditions of CMS in middle age
rats. Thus, this hormone reverted the decrement of DCX-cells caused
during normal aging. (C) 2017 Elsevier B.V. All rights reserved.
Filiaciones:
Herrera-Perez, J. J.:
Inst Nacl Psiquiatria Ramon de la Fuente Muniz, Direcc Invest Neurociencias, Farmacol Conductual, Calzada Mexico Xochimilco 101, Mexico City 14370, DF, Mexico
Martinez-Mota, L.:
Inst Nacl Psiquiatria Ramon de la Fuente Muniz, Direcc Invest Neurociencias, Farmacol Conductual, Calzada Mexico Xochimilco 101, Mexico City 14370, DF, Mexico
Jimenez-Rubio, G.:
Inst Nacl Psiquiatria Ramon de la Fuente Muniz, Direcc Invest Neurociencias, Farmacol Conductual, Calzada Mexico Xochimilco 101, Mexico City 14370, DF, Mexico
Ortiz-Lopez, L.:
Inst Nacl Psiquiatria Ramon de la Fuente Muniz, Subdirecc Invest Clin, Lab Neurogenesis, Calzada Mexico Xochimilco 101, Mexico City 14370, DF, Mexico
Cabrera-Munoz, E. A.:
Inst Nacl Psiquiatria Ramon de la Fuente Muniz, Subdirecc Invest Clin, Lab Neurogenesis, Calzada Mexico Xochimilco 101, Mexico City 14370, DF, Mexico
Galindo-Sevilla, N.:
Inst Nacl Perinatol, Dept Infectol & Inmunol, Montes Urales 800, Mexico City 11000, DF, Mexico
Zambrano, E.:
Inst Nacl Ciencias Med & Nutr Salvador Zubiran, Dept Anim Nutr, Ave Vasco de Quiroga 15, Mexico City 14080, DF, Mexico
Hernandez-Luis, F.:
Univ Nacl Autonoma Mexico, Fac Quim, Dept Farm, Circuito Exterior S-N, Mexico City 04510, DF, Mexico
Ramirez-Rodriguez, G. B.:
Inst Nacl Psiquiatria Ramon de la Fuente Muniz, Subdirecc Invest Clin, Lab Neurogenesis, Calzada Mexico Xochimilco 101, Mexico City 14370, DF, Mexico
Flores-Ramos, M.:
Catedrat Consejo Nacl Ciencia & Tecnol, Ave Insurgentes Sur 1582, Mexico City 03940, DF, Mexico
Inst Nacl Psiquiatria Ramon de la Fuente Muniz, Clin Trastornos Afect, Calzada Mexico Xochimilco 101, Mexico City 14370, DF, Mexico
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