Parasiticidal effect of synthetic bovine lactoferrin peptides on the enteric parasite Giardia intestinalis
Por:
Aguilar-Diaz, Hugo, Canizalez-Roman, Adrian, Nepomuceno-Mejia, Tomas, Gallardo-Vera, Francisco, Hornelas-Orozco, Yolanda, Nazmi, Kamran, Bolscher, Jan G. M., Carrero, Julio Cesar, Leon-Sicairos, Claudia, Leon-Sicairos, Nidia
Publicada:
1 feb 2017
Resumen:
Giardia intestinalis is the most common infectious protozoan parasite in
children. Despite the effectiveness of some drugs, the disease remains a
major worldwide problem. Consequently, the search for new treatments is
important for disease eradication. Biological molecules with
antimicrobial properties represent a promising alternative to combat
pathogens. Bovine lactoferrin (bLF) is a key component of the innate
host defense system, and its peptides have exhibited strong
antimicrobial activity. Based on these properties, we evaluated the
parasiticidal activity of these peptides on G. intestinalis.
Trophozoites were incubated with different peptide concentrations for
different periods of time, and the growth or viability was determined by
carboxyfluorescein-succinimidyl-diacetate-ester (CFDA) and propidium
iodide (PI) staining. Endocytosis of peptides was investigated by
confocal microscopy, damage was analyzed by transmission and scanning
electron microscopy, and the type of programmed cell death was analyzed
by flow cytometry. Our results showed that the LF peptides had
giardicidal activity. The LF peptides interacted with G. intestinalis
and exposure to LF peptides correlated with an increase in the
granularity and vacuolization of the cytoplasm. Additionally, the
formation of pores, extensive membrane disruption, and programmed cell
death was observed in trophozoites treated with LF peptides. Our results
demonstrate that LF peptides exhibit potent in vitro antigiardial
activity.
Filiaciones:
Aguilar-Diaz, Hugo:
Univ Autonoma Sinaloa, Fac Med, CIASaP, Culiacan 80246, Sinaloa, Mexico
Canizalez-Roman, Adrian:
Univ Autonoma Sinaloa, Fac Med, CIASaP, Culiacan 80246, Sinaloa, Mexico
Hosp Mujer, Dept Invest, Blvd Miguel Tamayo Espinoza Monteros S-N, Culiacan 80020, Sinaloa, Mexico
Nepomuceno-Mejia, Tomas:
Inst Nacl Salud Publ, Ctr Reg Invest Salud Publ, Calle 4a,Ave Norte Esquina Con Calle 19 Pte S-N, Tapachula 30700, Chiapas, Mexico
Gallardo-Vera, Francisco:
Univ Nacl Autonoma Mexico, Fac Med, Lab Inmunobiol, Dept Biol Celular & Tisular, Ciudad Univ, Mexico City 04510, DF, Mexico
Hornelas-Orozco, Yolanda:
Univ Nacl Autonoma Mexico, Inst Ciencias Mar & Limnol, Serv Acad Microscopia Elect Barrido, Mexico City 04510, DF, Mexico
Nazmi, Kamran:
Univ Amsterdam, Acad Ctr Dent Amsterdam, Dept Oral Biochem, NL-1081 LA Amsterdam, Netherlands
Vrije Univ Amsterdam, NL-1081 LA Amsterdam, Netherlands
Bolscher, Jan G. M.:
Univ Amsterdam, Acad Ctr Dent Amsterdam, Dept Oral Biochem, NL-1081 LA Amsterdam, Netherlands
Vrije Univ Amsterdam, NL-1081 LA Amsterdam, Netherlands
Carrero, Julio Cesar:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City 04510, DF, Mexico
Leon-Sicairos, Claudia:
Univ Autonoma Sinaloa, Fac Ciencias Quim Biol, Ave Americas & Josefa Ortiz,Ciudad Univ, Culiacan 80030, Sinaloa, Mexico
Leon-Sicairos, Nidia:
Univ Autonoma Sinaloa, Fac Med, CIASaP, Culiacan 80246, Sinaloa, Mexico
Hosp Pediat Sinaloa, Dept Invest, Blvd Constituc S-N, Culiacan 80200, Sinaloa, Mexico
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