Differentially Expressed Long Non-Coding RNAs Were Predicted to Be Involved in the Control of Signaling Pathways in Pediatric Astrocytoma
Por:
Ruiz Esparza-Garrido R., Rodríguez-Corona J.M., López-Aguilar J.E., Rodríguez-Florido M.A., Velázquez-Wong A.C., Viedma-Rodríguez R., Salamanca-Gómez F., Velázquez-Flores M.Á.
Publicada:
1 oct 2017
Categoría:
Cellular and Molecular Neuroscience
Resumen:
Expression changes for long non-coding RNAs (lncRNAs) have been
identified in adult glioblastoma multiforme (GBM) and in a mixture of
adult and pediatric astrocytoma. Since adult and pediatric astrocytomas
are molecularly different, the mixture of both could mask specific
features in each. We determined the global expression patterns of
lncRNAs and messenger RNA (mRNAs) in pediatric astrocytoma of different
histological grades. Transcript expression changes were determined with
an HTA 2.0 array. lncRNA interactions with microRNAs and mRNAs were
predicted by using an algorithm and the LncTar tool, respectively.
Interactomes were constructed with the HIPPIE database and visualized
with the Cytoscape platform. The array showed expression changes in 156
and 207 lncRNAs in tumors (versus the control) and in pediatric GBM
(versus low-grade astrocytoma), respectively. Predictions identified
lncRNAs that have putative microRNA binding sites, which might suggest
that they function as sponges in these tumors. Also, lncRNAs were shown
to interact with many mRNAs, such as Pleckstrin homology-like domain,
family A, member 1 (PHLDA1) and sulfatase 2 (SULF2). For example, qPCR
found long intergenic non-coding RNA regulator of reprogramming
(linc-RoR) expression levels upregulated in pediatric GBM when they were
compared with control tissues or with low-grade tumors. Meanwhile,
PHLDA1 and ELAV-like RNA binding protein 1 (ELAV1) showed expression
changes in tumors relative to the control. Our data showed many lncRNAs
with expression changes in pediatric astrocytoma, which might be
involved in the regulation of different signaling pathways.
Filiaciones:
Functional Genomics Laboratory, Unit of Human Genetics Research, Children’s Hospital, “Dr. Silvestre Frenk Freund,” National Medical Center Century XXI, Mexican Institute of Social Security (IMSS), Mexico City, Mexico
Oncology Department, Children’s Hospital, “Dr. Silvestre Frenk Freund,” National Medical Center Century XXI, Mexican Institute of Social Security (IMSS), Mexico City, Mexico
Developmental Biology Laboratory, Unit of Morphology and Cellular Function, Faculty of Higher Education Iztacala, National Autonomous University of Mexico, Tlalnepantla, State of Mexico, Mexico
Health Research Coordination, National Medical Center Century XXI, Mexican Institute of Social Security (IMSS), Mexico City, Mexico
Functional Genomics Laboratory, Unit of Human Genetics Research, Children’s Hospital, “Dr. Silvestre Frenk Freund,” National Medical Center Century XXI, Mexican Institute of Social Security (IMSS), Mexico City, 06720, Mexico
Oncology Department, Children’s Hospital, “Dr. Silvestre Frenk Freund,” National Medical Center Century XXI, Mexican Institute of Social Security (IMSS), Mexico City, 06720, Mexico
Developmental Biology Laboratory, Unit of Morphology and Cellular Function, Faculty of Higher Education Iztacala, National Autonomous University of Mexico, Tlalnepantla, State of Mexico 54090, Mexico
Health Research Coordination, National Medical Center Century XXI, Mexican Institute of Social Security (IMSS), Mexico City, 06720, Mexico
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