Variation in pestivirus growth in testicle primary cell culture is more dependent on the individual cell donor than cattle breed
Por:
Weber, Matheus N., Bauermann, Fernando V., Gomez-Romero, Ninnet, Herring, Andy D., Canal, Cludio W., Neill, John D., Ridpath, Julia F.
Publicada:
1 mar 2017
Resumen:
The causes of bovine respiratory disease complex (BRDC) are multifactorial and include infection with both viral and bacterial pathogens. Host factors are also involved as different breeds of cattle appear to have different susceptibilities to BRDC. Infection with bovine pestiviruses, including bovine viral diarrhea virus 1 (BVDV1), BVDV2 and ‘HoBi’-like viruses, is linked to the development of BRDC. The aim of the present study was to compare the growth of different bovine pestiviruses in primary testicle cell cultures obtained from taurine, indicine and mixed taurine and indicine cattle breeds. Primary cells strains, derived from testicular tissue, were generated from three animals from each breed. Bovine pestivirus strains used were from BVDV-1a, BVDV-1b, BVDV-2a and ‘HoBi’-like virus. Growth was compared by determining virus titers after one passage in primary cells. All tests were run in triplicate. Virus titers were determined by endpoint dilution and RT-qPCR. Statistical analysis was performed using one way analysis of variance (ANOVA) followed by the Tukey’s Multiple Comparison Test (P?0.05). Significant differences in virus growth did not correlate with cattle breed. However, significant differences were observed between cells derived from different individuals regardless of breed. Variation in the replication of virus in primary cell strains may reflect a genetic predisposition that favors virus replication. © 2016, Springer Science+Business Media Dordrecht (outside the USA).
Filiaciones:
Weber, Matheus N.:
Univ Fed Rio Grande do Sul, Fac Vet, Lab Virol, Porto Alegre, RS, Brazil
Bauermann, Fernando V.:
NADC, Ruminant Dis & Immunol Res Unit, USDA ARS, 1920 Dayton Ave,POB 70, Ames, IA 50010 USA
Gomez-Romero, Ninnet:
Univ Nacl Autonoma Mexico, FMVZ, Lab Vacunol & Constatac, Mexico City 04510, DF, Mexico
Herring, Andy D.:
Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA
Neill, John D.:
NADC, Ruminant Dis & Immunol Res Unit, USDA ARS, 1920 Dayton Ave,POB 70, Ames, IA 50010 USA
Ridpath, Julia F.:
NADC, Ruminant Dis & Immunol Res Unit, USDA ARS, 1920 Dayton Ave,POB 70, Ames, IA 50010 USA
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