A benzimidazole derivative (RCB20) in vitro induces an activation of energetic pathways on Taenia crassiceps (ORF strain) cysticerci
Por:
Fraga, Carolina Miguel, da Costa, Tatiane Luiza, de Castro, Ana Maria, Reynoso-Ducoing, Olivia, Ambrosio, Javier, Hernandez-Campos, Alicia, Castillo, Rafael, Vinaud, Marina Clare
Publicada:
1 ene 2017
Resumen:
Human cysticercosis caused by Taenia crassiceps is unusual; however, it
is an useful experimental model for cysticercosis studies. Benzimidazole
derivatives are important antihelminthic drugs widely used against
helminths. A novel compound 6-chloro-5-(1-naphthyloxy)
-2-(trifluoromethyl)-1H-benzimidazole (RCB20) is a benzimidazole
derivative less polar and more lipophilic. The aim of this study was to
detect the effect of the RCB20 on the in vitro energetic metabolism of
T. crassiceps cysticerci. For this, products of the metabolism both
produced and secreted/excreted (S/E) by the parasite were detected
through spectrophotometry and high performance liquid chromatography
after exposure to 6.5 and 13 mu M of RCB20 and albendazole sulfoxide
(ABZSO). There was a gradual increase in the concentrations of glucose
not uptaken by parasites exposed to both concentrations RCB20 and ABZSO.
There was a higher concentration of all the organic acids related to the
tricarboxilic acid cycle int the parasites exposed to RCB20. The
structural differences between RCB20 and ABZSO result in different
targets within the parasite and in a greater induction of the energetic
pathways, such as the glycolysis and the TCA cycle. RCB20 is a good
candidate as a substitute for anthelminthic benzimidazoles due to a
differentiated site of action with similar outcome. (C) 2016 Elsevier
Inc. All rights reserved.
Filiaciones:
Fraga, Carolina Miguel:
Laboratory of Studies of the Host-parasite Relationship, Tropical Pathology and Public Health Institute, Federal University of Goias, Brazil
Univ Fed Goias, Lab Studies Host Parasite Relationship, Trop Pathol & Publ Hlth Inst, Rua 235 S-N, BR-74605050 Goiania, Go, Brazil
da Costa, Tatiane Luiza:
Laboratory of Studies of the Host-parasite Relationship, Tropical Pathology and Public Health Institute, Federal University of Goias, Brazil
Univ Fed Goias, Lab Studies Host Parasite Relationship, Trop Pathol & Publ Hlth Inst, Rua 235 S-N, BR-74605050 Goiania, Go, Brazil
de Castro, Ana Maria:
Laboratory of Studies of the Host-parasite Relationship, Tropical Pathology and Public Health Institute, Federal University of Goias, Brazil
Univ Fed Goias, Lab Studies Host Parasite Relationship, Trop Pathol & Publ Hlth Inst, Rua 235 S-N, BR-74605050 Goiania, Go, Brazil
Reynoso-Ducoing, Olivia:
Laboratorio de Biologia del Citoesqueleto, Departamento de Microbiologia y Parasitologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico (UNAM), México, DF, Mexico
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Lab Biol Citoesqueleto, Mexico City 04510, DF, Mexico
Ambrosio, Javier:
Laboratorio de Biologia del Citoesqueleto, Departamento de Microbiologia y Parasitologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico (UNAM), México, DF, Mexico
Univ Nacl Autonoma Mexico, Dept Microbiol & Parasitol, Fac Med, Lab Biol Citoesqueleto, Mexico City 04510, DF, Mexico
Hernandez-Campos, Alicia:
Facultad de Química, Departamento de Farmacia, Universidad Nacional Autónoma de México (UNAM), México, DF, Mexico
Univ Nacl Autonoma Mexico, Dept Farm, Fac Quim, Mexico City 04510, DF, Mexico
Castillo, Rafael:
Facultad de Química, Departamento de Farmacia, Universidad Nacional Autónoma de México (UNAM), México, DF, Mexico
Univ Nacl Autonoma Mexico, Dept Farm, Fac Quim, Mexico City 04510, DF, Mexico
Vinaud, Marina Clare:
Laboratory of Studies of the Host-parasite Relationship, Tropical Pathology and Public Health Institute, Federal University of Goias, Brazil
Univ Fed Goias, Lab Studies Host Parasite Relationship, Trop Pathol & Publ Hlth Inst, Rua 235 S-N, BR-74605050 Goiania, Go, Brazil
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