Identification of the infectious source of an unusual outbreak of histoplasmosis, in a hotel in Acapulco, state of Guerrero, Mexico
Por:
Taylor M.L., Ruíz-Palacios G.M., Reyes-Montes M.D.R., Rodríguez-Arellanes G., Carreto-Binaghi L.E., Duarte-Escalante E., Hernández-Ramírez A., Pérez A., Suárez-Alvarez R.O., Roldán-Aragón Y.A., Romero-Martínez R., Sahaza-Cardona J.H., Sifuentes-Osornio J., Soto-Ramírez L.E., Peña-Sandoval G.R.
Publicada:
1 ene 2005
Resumen:
Three isolates of Histoplasma capsulatum were identified from mice lung, liver, and spleen inoculated with soil samples of the X hotel's ornamental potted plants that had been fertilized with organic material known as compost. The presence of H. capsulatum in the original compost was detected using the dot-enzyme-linked immunosorbent assay. Nested-PCR, using a specific protein Hcp100 coding gene sequence, confirmed the fungal identification associated with an unusual histoplasmosis outbreak in Acapulco. Although, diversity between the H. capsulatum isolate from the hotel and some clinical isolates from Guerrero (positive controls) was observed using random amplification of polymorphic DNA based-PCR, sequence analyses of H-anti and ole fragment genes revealed a high homology (92-99%) between them. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Filiaciones:
Laboratorio de Inmunología de Hongos, Departamento de Microbiología Y Parasitología, Ciudad Universitaria s/n, México DF 04510, Mexico
Departamento de Infectología, Instituto Nacional de Ciencias Médicas Y Nutrición Salvador Zubirán, México DF 14000, Mexico
Departamento de Biología Celular Y Tisular, Facultad de Medicina UNAM, México DF 04510, Mexico
All Open Access; Bronze
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