Participation of a sialic acid-specific lectin from freshwater prawn Macrobrachium rosenbergii hemocytes in the recognition of non-self cells
Por:
Vázquez L., Maldonado G., Agundis C., Pérez A., Cooper E.L., Zenteno E.
Publicada:
1 ene 1997
Resumen:
Phagocytic activity of circulating hemocytes from freshwater prawns is mediated by a small group of granulocytes. Recognition of nonself cells by these cells seems to be mediated by two independent mechanisms: specific, via O-acetylsialic acid, as well as N-acetylated sugars on recognized cells and a nonspecific one. Both mechanisms show differences in their optimal temperature and time differences for activity. Hemocytes with phagocytic activity interact positively with rabbit IgG raised against the serum lectin. Attempts to elucidate the nature of the membrane-lectin on hemocytes performed by electroimmunotransfer blot assays on cell lysates indicates the presence of two major proteins with a molecular weight of 68-72 kDa. Electron microscopy revealed a regular distribution of lectin on hemocyte plasma membranes. Our results suggest the active participation of a membrane lectin in the recognition of nonself.
Filiaciones:
Vázquez L.:
Laboratorio de Lectinas, Ctro. de Invest. Químicas, Univ. Autonoma del Estado de Morelos, Cuernavaca Morelos, Mexico
Depto. Bioquimica, Facultad de Medicina UNAM, P.O. Box 70159, 04510 México, D.F., Mexico
Maldonado G.:
Departamento de Bioquímica, Inst. Nac. de Enferm. Respiratorias, Tlalpan, México D.F., Mexico
Agundis C.:
Departamento de Bioquímica, Inst. Nac. de Enferm. Respiratorias, Tlalpan, México D.F., Mexico
Pérez A.:
Depto. de Biol. Celular y Tisular, Facultad de Medicina UNAM, México 04510 D.F., Mexico
Cooper E.L.:
Laboratory of Comparative Immunology, Department of Neurobiology, UCLA Medical Center, Los Angeles, CA 90095-1736, United States
Zenteno E.:
Laboratorio de Inmunologia, Departamento de Bioquímica, Facultad de Medicina UNAM, México 04510 D.F., Mexico
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