Abnormal distribution of E-cadherin and ß-catenin in different histologic types of cancer of the uterine cervix
Por:
Rodríguez-Sastre M.A., González-Maya L., Delgado R., Lizano M., Tsubaki G., Mohar A., García-Carrancá A.
Publicada:
1 ene 2005
Resumen:
Objective. The goal of this study was to analyze the cellular distribution and possible alterations of ?-catenin and E-cadherin proteins in different histologic types of uterine cervical cancer and precursor lesions, compared to normal controls. Methods. We performed an immunochemical staining analysis of the cellular distribution of ?-catenin and E-cadherin proteins in biopsy samples from 20 normal exocervical squamous epithelium, 43 premalignant lesions, and a large series of 126 invasive tumors of different histologic types that included 68 squamous carcinomas, 31 adenosquamous carcinomas, and 27 adenocarcinomas. Statistical significance was evaluated by the chi-square or Fisher's Exact test. Results. We observed ?-catenin abnormally distributed in the cytoplasm of 62% of premalignant lesions and more than 70% of invasive cancers, statistically significant when compared with normal tissue (P < 0.05). Similarly, we found that E-cadherin exhibit a significant abnormal distribution in the cytoplasm of 58% of premalignant lesions (P < 0.05) and in more than 71% of squamous carcinoma and adenosquamous carcinoma when compared with normal tissue (P < 0.05). We found no differences in the distribution of E-cadherin between adenocarcinomas compared with control samples. Interestingly, we found that both, ?-catenin and E-cadherin, were absent in the membrane of nearly 40% premalignant lesions. Nuclear staining of ?-catenin was rarely seen in any cases, contrary to what has been reported for this and other neoplasias. Conclusion. Our findings indicate that cellular alterations of both ?-catenin and E-cadherin are frequent in tumors of the uterine cervix of different histologic types, and support a role for these proteins in cervical cancer development. © 2005 Elsevier Inc. All rights reserved.
Filiaciones:
Rodríguez-Sastre M.A.:
Depto. Biol. Molec. Y Biotecnologia, Inst. de Invest. Bioméd.-UNAM, México D.F., Mexico
Div. de Investigación, Inst. Nac. de Cancerologia-SSA, México D.F., Mexico
González-Maya L.:
Facultad de Farmacia, UAEM, Cuernavaca, Morelos, Mexico
Delgado R.:
Departamento de Patología, Inst. Nac. de Cancerologia-SSA, México D.F., Mexico
Lizano M.:
Div. de Investigación, Inst. Nac. de Cancerologia-SSA, México D.F., Mexico
Tsubaki G.:
Departamento de Ginecología, Hospital Manuel Gea González, México D.F., Mexico
Mohar A.:
Depto. Med. Genomica Y Toxicol. A., Inst. de Invest. Bioméd.-UNAM, México D.F., Mexico
Div. de Investigación, Inst. Nac. de Cancerologia-SSA, México D.F., Mexico
García-Carrancá A.:
Depto. Biol. Molec. Y Biotecnologia, Inst. de Invest. Bioméd.-UNAM, México D.F., Mexico
Div. de Investigación, Inst. Nac. de Cancerologia-SSA, México D.F., Mexico
Div. de Investigación, Inst. Nac. de Cancerología, SSA, Avenida San Fernando No. 22, Tlalpan, México D. F., Mexico
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