Peptide mimotopes of Mycobacterium tubercolosis carbohydrate immunodeterminants
Por:
Gevorkian G., Segura E., Acero G., Palma J.P., Espitia C., Manoutcharian K., López-Marín L.M.
Publicada:
1 ene 2005
Resumen:
Cell-surface saccharides of Mycobacterium tuberculosis appear to be crucial factors in tuberculosis pathogenicity and could be useful antigens in tuberculosis immunodiagnosis. In the present study, we report the successful antigenic and immunogenic mimicry of mannose-containing cell-wall compounds of M. tuberculosis by dodecamer peptides identified by phage-display technology. Using a rabbit antiserum raised against M. tuberculosis cell-surface saccharides as a target for biopanning, peptides with three different consensus sequences were identified, Phage-displayed and chemically synthesized peptides bound to the anti-carbohydrate antiserum. Rabbit antibodies elicited against the peptide QEPLMGTVPIRAGGGS recognize the mannosylated M. tuberculosis cell-wall antigens arabinomannan and lipoarabinomannan, and the glycosylated recombinant protein alanine/ proline-rich antigen. Furthermore, antibodies were also able to react with mannan from Saccharomyces cerevisiae, but not with phosphatidylinositol dimannosides or arabinogalactan from mycobacteria. These results suggest that the immunogenic peptide mimics oligomannosidic epitopes. Interestingly, this report provides evidence that, in contrast with previously known carbohydrate mimotopes, no aromatic residues are necessary in a peptide sequence for mimicking unusual glycoconjugates synthesized by mycobacteria. The possible usefulness of the identified peptide mimotopes as surrogate reagents for immunodiagnosis and for the study of functional roles of the native non-peptide epitopes is discussed. © 2005 Biochemical Society.
Filiaciones:
Gevorkian G.:
Departamento de Inmunología, Inst. de Investigaciones Biomedicas, Univ. Nacional Autonoma de Mexico, Apdo. Postal 70-228, Coyoacán 04510, Mexico
Segura E.:
Departamento de Inmunología, Inst. de Investigaciones Biomedicas, Univ. Nacional Autonoma de Mexico, Apdo. Postal 70-228, Coyoacán 04510, Mexico
Acero G.:
Departamento de Inmunología, Inst. de Investigaciones Biomedicas, Univ. Nacional Autonoma de Mexico, Apdo. Postal 70-228, Coyoacán 04510, Mexico
Palma J.P.:
Departamento de Inmunología, Inst. de Investigaciones Biomedicas, Univ. Nacional Autonoma de Mexico, Apdo. Postal 70-228, Coyoacán 04510, Mexico
Espitia C.:
Departamento de Inmunología, Inst. de Investigaciones Biomedicas, Univ. Nacional Autonoma de Mexico, Apdo. Postal 70-228, Coyoacán 04510, Mexico
Manoutcharian K.:
Depto. de Biol. Molec. y Biotecnol., Inst. de Investigaciones Biomedicas, Univ. Nacional Autonoma de Mexico, Apdo. Postal 70-228, Coyoacán 04510, Mexico
López-Marín L.M.:
Departamento de Inmunología, Inst. de Investigaciones Biomedicas, Univ. Nacional Autonoma de Mexico, Apdo. Postal 70-228, Coyoacán 04510, Mexico
All Open Access; Green
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