Evidence supporting a major promoter in the Trypanosoma cruzi rRNA gene
Por:
Figueroa-Angulo E., Martínez-Calvillo S., López-Villaseñor I., Hernández R.
Publicada:
1 ene 2003
Resumen:
Two clearly separated transcription start points (tsp) have been reported within the Trypanosoma cruzi rDNA (DNA encoding rRNA) gene spacer region. These sites are separated by 270 bp, a distance compatible with the occurrence of two core promoters. To characterize the individual participation of these two elements, a deletion analysis was carried out. Different versions of the promoter regions were assayed in a transient transfection analysis of epimastigotes, using the chloramphenicol acetyl transferase gene (cat) as a reporter. The data indicate that the so-called distal tsp-associated region (relative to the small subunit rRNA 5? terminus coding region) comprises most (80%) if not all of the observed activity. In addition, an associated locus specific repeated element showed a modest upregulating activity, since its presence stimulated the cat reporter gene by about 20%. The data here presented should be valuable in the design of expression vectors for T. cruzi, where the rRNA gene promoter has been an important functional element. © 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Filiaciones:
Figueroa-Angulo E.:
Inst. de Invest. Biomédicas, Univ. Nac. Auton. de México, Apartado Postal 70-228, 04510 México D.F., Mexico
Martínez-Calvillo S.:
Inst. de Invest. Biomédicas, Univ. Nac. Auton. de México, Apartado Postal 70-228, 04510 México D.F., Mexico
Seattle Biomed. Research Institute, 4 Nickerson Street, Seattle, WA 98109-1651, United States
López-Villaseñor I.:
Inst. de Invest. Biomédicas, Univ. Nac. Auton. de México, Apartado Postal 70-228, 04510 México D.F., Mexico
Hernández R.:
Inst. de Invest. Biomédicas, Univ. Nac. Auton. de México, Apartado Postal 70-228, 04510 México D.F., Mexico
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