Mimotopes of conformational epitopes in fibrillar ßamyloid
Por:
Gevorkian G., Petrushina I., Manoutcharian K., Ghochikyan A., Acero G., Vasilevko V., Cribbs D.H., Agadjanyan M.G.
Publicada:
1 ene 2004
Resumen:
In Alzheimer's disease (AD) ßamyloid peptide accumulates in the brain in different forms including fibrils. Amyloid fibrils could be recognized as foreign by the mature immune system since they are not present during its development. Thus, using mouse antisera raised against the fibrillar form of Aß 42, we have screened two phage peptide libraries for the presence of foreign conformational mimotopes of Aß. Antisera from wild type animals recognized predominately peptides with the EFRH motif from Aß 42 sequence, whereas amyloid precursor protein (APP) transgenic mice recognized mainly phage clones that mimic epitopes (mimotopes) within the fibrillar Aß 42 but lack sequence homology with this peptide. © 2004 Elsevier B.V. All rights reserved.
Filiaciones:
Gevorkian G.:
Inst. de Invest. Biomédicas, Univ. Nac. Autonoma de México, México, Mexico
Petrushina I.:
Inst. for Brain Aging and Dementia, University of California Irvine, Irvine, CA 92697, United States, United States
Manoutcharian K.:
Inst. de Invest. Biomédicas, Univ. Nac. Autonoma de México, México, Mexico
Ghochikyan A.:
Department of Immunology, The Institute for Molecular Medicine, 16371 Gothard St., Huntington B., United States
Acero G.:
Inst. de Invest. Biomédicas, Univ. Nac. Autonoma de México, México, Mexico
Vasilevko V.:
Inst. for Brain Aging and Dementia, University of California Irvine, Irvine, CA 92697, United States, United States
Cribbs D.H.:
Inst. for Brain Aging and Dementia, University of California Irvine, Irvine, CA 92697, United States, United States
Agadjanyan M.G.:
Department of Immunology, The Institute for Molecular Medicine, 16371 Gothard St., Huntington B., United States
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