Participation of serum and membrane lectins on the oxidative burst regulation in Macrobrachium rosenbergii hemocytes
Por:
Sierra C., Lascurain R., Pereyra A., Guevara J., Martínez G., Agundis C., Zenteno E., Vázquez L.
Publicada:
1 ene 2005
Resumen:
Using a spectrophotometric NBT reduction assay and phagocytosis, we identified that production of superoxide anions and phagocytic activity of hemocytes from Macrobrachium rosenbergii were significantly higher in the presence of rat, rabbit, and chicken erythrocytes than with human, pig, or horse erythrocytes. Hemocytes stimulated with MrL, MrLMab, or PMA increased 4.7, 5.1, and 6.1 fold, respectively, the oxidative response as compared to non-stimulated hemocytes. MrLMab together with MrL increased 5.7 fold the oxidative capacity of hemocytes as compared to non-stimulated cells. These effects were inhibited with 100 mM GalNAc, GlcNAc, or Neu5Ac and 0.2 ?M of sialylated submaxillary gland mucin and fetuin. Piroxicam inhibited (P<0.05) the production of O 2 - induced by MrL, whereas iodoacetamide inhibited the effect of MrLMAb (P<0.05) in a dose-dependent manner. Our results suggest that MrLMab might activate the oxidative burst through the metabolism of glucose as opposed to MrL which utilizes NADPH-independent mechanisms, very probably through pro-inflammatory metabolites. © 2004 Elsevier Ltd. All rights reserved.
Filiaciones:
Sierra C.:
Laboratorio de Lectinas, Ctro. de Invest. Químicas, Univ. Auton. Estado Morelos, Ave. U., Mexico
Lascurain R.:
Departamento de Bioquímica, Inst. de Enfermedades Respiratorias, Mexico, DF 10245, Mexico, Spain
Pereyra A.:
Departamento de Bioquímica, Inst. de Enfermedades Respiratorias, Mexico, DF 10245, Mexico, Spain
Guevara J.:
Lab. de Enferm. Neurodegenerativas, Inst. Nac. de Neurol. Y Neurocirugia, Mexico, DF 14269, Mexico, Mexico
Martínez G.:
Laboratorio de Inmunología, Departamento de Bioquímica, Fac. Med., UNAM, Mex., DF 04510, M
Agundis C.:
Laboratorio de Inmunología, Departamento de Bioquímica, Fac. Med., UNAM, Mex., DF 04510, M
Zenteno E.:
Laboratorio de Inmunología, Departamento de Bioquímica, Fac. Med., UNAM, Mex., DF 04510, M
Vázquez L.:
Laboratorio de Lectinas, Ctro. de Invest. Químicas, Univ. Auton. Estado Morelos, Ave. U., Mexico
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