Use of in vitro assays to assess the potential antigenotoxic and cytotoxic effects of saffron (Crocus sativus L.)
Por:
Abdullaev F.I., Riverón-Negrete L., Caballero-Ortega H., Manuel Hernández J., Pérez-López I., Pereda-Miranda R., Espinosa-Aguirre J.J.
Publicada:
1 ene 2003
Resumen:
Saffron is harvested from the dried, dark red stigmas of Crocus sativus L. flowers. It is used as a spice for flavoring and coloring food and as a perfume. It is often used for treating several diseases. We assessed the antimutagenic, comutagenic and cytotoxic effects of saffron and its main ingredients using the Ames/Salmonella test system, two well known mutagens (BP, 2AA), the in vitro colony formation assay and four different cultured human normal (CCD-18Lu) and malignant (HeLa, A-204 and HepG2) cells. When only using the TA98 strain in the Ames/Salmonella test system, saffron showed non-mutagenic, as well as non-antimutagenic activity against BP-induced mutagenicity, and demonstrated a dose-dependent co-mutagenic effect on 2-AA-induced mutagenicity. The saffron component responsible for this unusual comutagenic effect was safranal. In the in vitro colony formation test system, saffron displayed a dose-dependent inhibitory effect only against human malignant cells. All isolated carotenoid ingredients of saffron demonstrated cytotoxic activity against in vitro tumor cells. Saffron crocin derivatives possessed a stronger inhibitory effect on tumor cell colony formation. Overall, these results suggest that saffron itself, as well as its carotenoid components might be used as potential cancer chemopreventive agents. © 2003 Elsevier Ltd. All rights reserved.
Filiaciones:
Abdullaev F.I.:
Inst. Nacional de Pediatría, Torre de Investigación, Avenida del Imán # 1, 04530 México D.F., Mexico
Riverón-Negrete L.:
Inst. Nacional de Pediatría, Torre de Investigación, Avenida del Imán # 1, 04530 México D.F., Mexico
Caballero-Ortega H.:
Inst. Nacional de Pediatría, Torre de Investigación, Avenida del Imán # 1, 04530 México D.F., Mexico
Manuel Hernández J.:
Inst. Nacional de Pediatría, Torre de Investigación, Avenida del Imán # 1, 04530 México D.F., Mexico
Pérez-López I.:
Inst. Nacional de Pediatría, Torre de Investigación, Avenida del Imán # 1, 04530 México D.F., Mexico
Pereda-Miranda R.:
Facultad De Quimica, UNAM, Mexico, Mexico
Espinosa-Aguirre J.J.:
Inst. Nacional de Pediatría, Torre de Investigación, Avenida del Imán # 1, 04530 México D.F., Mexico
Inst. de Invest. Biomédicas, UNAM, México, Mexico
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