Purification of the receptor for the N-acetyl-d-glucosamine specific adhesin of Mannheimia haemolytica from bovine neutrophils
Por:
De la Mora A., Suárez-Güemes F., Trigo F., Gorocica P., Solórzano C., Slomianny M.-C., Agundis C., Pereyra M.A., Zenteno E.
Publicada:
1 ene 2007
Resumen:
The GlcNAc-specific adhesin from Mannheimia haemolytica (MhA) has been shown to participate in pathogenicity of mannheimiosis due to its capacity to adhere to tracheal epithelial cells and activate the oxidative burst of bovine neutrophils. In this work, we purified the MhA receptor from bovine neutrophils (MhAr) by affinity chromatography on MhA-Sepharose. The MhAr, which corresponded to approximately 2% of the protein from cell lysate, is a glycoprotein mainly composed of Glu, Ala, Ser, Gly, and Asp, without cysteine. The glycan portion, which corresponds to 20% by weight, is composed of GalNAc, GlcNAc, Man, Gal, and NeuAc. The receptor is a 165-kDa glycoprotein, as determined by molecular sieve chromatography under native conditions; SDS-PAGE analysis shows a heterodimer of 83 and 80 kDa subunits. This work suggests that the GlcNAc-containing receptor plays a relevant role by activating bovine neutrophils through non-opsonic mechanisms. © 2007 Elsevier B.V. All rights reserved.
Filiaciones:
De la Mora A.:
Laboratorio de Patología, Instituto de Investigaciones en Ciencias Veterinarias, Universidad Autónoma de Baja California, Mexicali, BC, Mexico
Suárez-Güemes F.:
Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Mexico
Trigo F.:
Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Mexico
Gorocica P.:
Departamento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, Secretaría de Salud, Mexico
Solórzano C.:
Laboratoire de Chimie Biologique, la Université des Sciences et Technologies de Lille, UMR, Villeneuve d'Ascq, 59655 Cedex, France
Slomianny M.-C.:
Laboratoire de Chimie Biologique, la Université des Sciences et Technologies de Lille, UMR, Villeneuve d'Ascq, 59655 Cedex, France
Agundis C.:
Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de Mexico, P. O. Box 70159, 04510 México, D.F., Mexico
Pereyra M.A.:
Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de Mexico, P. O. Box 70159, 04510 México, D.F., Mexico
Zenteno E.:
Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de Mexico, P. O. Box 70159, 04510 México, D.F., Mexico
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