Organogenesis and somatic embryogenesis in Ariocarpus kotschoubeyanus (Lem.) K. Schum. (Cactaceae), an endemic and endangered Mexican species
Por:
Moebius-Goldammer K.G., Mata-Rosas M., Chávez-Avila V.M.
Publicada:
1 ene 2003
Resumen:
Shoots by direct and indirect organogenesis and somatic embryos were induced from tubercles excised from Ariocarpus kotschoubeyanus seeds germinated in vitro. Shoot formation was greatest (6.3 per explant) when explants were cultured on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BA; 13.3 ?M) and ?-naphthaleneacetic acid (NAA; 5.4 ?M). Individualized shoots were rooted in half-strength MS; the addition of activated charcoal (1 g l-1) and the use of sun cap closures to seal containers improved the rooting of shoots. Nearly 20% of the explants produced somatic embryos on media containing combinations of BA (8.9-22.2 ?M) and NAA (0.5-5.4 ?M). The establishment of plantlets in soil presented no significant problems. In vitro culture is a useful option for mass propagation of A. kotschoubeyanus and contributes to its conservation.
Filiaciones:
Moebius-Goldammer K.G.:
Jardin Botanico del Inst. de Biol., Univ. Nac. Auton. de Mex., D.F. 04510, Mexico, Mexico
Mata-Rosas M.:
Laboratorio de Cultivo de Tejidos, Jardin Botanico Francisco Javier C., Instituto de Ecología, Apdo. Postal 63, Xalapa, Veracruz, 91070, Mexico
Chávez-Avila V.M.:
Jardin Botanico del Inst. de Biol., Univ. Nac. Auton. de Mex., D.F. 04510, Mexico, Mexico
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