Unexpected immunoresponse to Gal and APA antigens in diabetic type 1 patients receiving neonatal pig islets after 6 years
Por:
Valdés-González R.A., Dorantes L.M., Garibay G.N., Bracho-Blanchet E., Dávila-Pérez R., Terán L., Ormsby C.E., Ayala-Sumuano J.-T., Copeman L., White D.J.G.
Publicada:
1 ene 2007
Resumen:
Cotransplantation of porcine islets and Sertoli cells into preimplanted subcutaneous devices improve metabolic control in type 1 diabetic patients, and survive grafted for more than 4 years. We report here, further assessment of the endocrine and porcine nature of the surviving cells and the immune responses elicited toward Gal ?(1,3)-Gal ?(1,4)-GlcNAc (Gal) antigen in patients who received a second and third transplants. No immunosuppressive drugs were administered. We were able to immunostain insulin- and glucagon-positive cells in all biopsies of patients and Sertoli cell markers in 60.9% of biopsies. Additionally, all biopsies tested, amplified the porcine COII gene. Patients demonstrated an increase in antipig antibodies in response to the first transplant with a decreasing response toward the second and third transplants. In all transplants, the IgG levels promptly returned to basal values after 3-4 months. The long-term survival of porcine cells and the reduced humoral immune response to multiple transplants indicate a form of tolerance. We have not been able to find CD25-positive cells, indicating that it is probably an immune accommodation of the graft. © 2007 Springer Science+Business Media, LLC.
Filiaciones:
Valdés-González R.A.:
Department of Surgery, Faculty of Medicine, UNAM, Mexico City, Mexico
Laboratory of Xenotransplantation, Children Hospital of Mexico Federico Gómez, Mexico City, Mexico
Hospital Ángeles del Pedregal Consultorio S-19, C. S. T. #1055, Col H. de Padierna, México, D.F., Mexico
Dorantes L.M.:
Department of Endocrinology, Children Hospital of Mexico Federico Gomez, Mexico City, Mexico
Garibay G.N.:
Department of Endocrinology, Children Hospital of Mexico Federico Gomez, Mexico City, Mexico
Bracho-Blanchet E.:
Department of Surgery, Children Hospital of Mexico Federico Gomez, Mexico City, Mexico
Dávila-Pérez R.:
Department of Surgery, Children Hospital of Mexico Federico Gomez, Mexico City, Mexico
Terán L.:
Laboratory of Xenotransplantation, Children Hospital of Mexico Federico Gómez, Mexico City, Mexico
Ormsby C.E.:
Laboratory of Xenotransplantation, Children Hospital of Mexico Federico Gómez, Mexico City, Mexico
Ayala-Sumuano J.-T.:
Laboratory of Xenotransplantation, Children Hospital of Mexico Federico Gómez, Mexico City, Mexico
Copeman L.:
Robarts Research Institute, University of Western Ontario, London, Ont., Canada
White D.J.G.:
Robarts Research Institute, University of Western Ontario, London, Ont., Canada
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