Salt and stress synergize H. pylori-induced gastric lesions, cell proliferation, and p21 expression in Mongolian gerbils
Por:
Gamboa-Dominguez A., Ubbelohde T., Saqui-Salces M., Romano-Mazzoti L., Cervantes M., Domínguez-Fonseca C., De La Luz Estreber M., Ruíz-Palacios G.M.
Publicada:
1 ene 2007
Resumen:
Our aim was to determine if salt and stress enhance Helicobacter pylori (Hp) lesions in Meriones unguiculatus. Two hundred seventy-eight pathogen-free gerbils were allocated to seven groups: Hp-Sydney strain (45), 8% higher-salt diet (38), stress (60% space reduction/water immersion; 36), Hp + salt (33), Hp + stress (34), N-methyl-N-nitro-N-nitrosoguanidine (34), and sham (58). Gerbils were sacrificed at 1 week (67), 12 weeks (73), 52 weeks (65), and 68 weeks (73). Sydney, Padova, and Lauren classifications were blindly used. Proliferation, p53, p21, and apoptosis were assessed. Follicular active gastritis (grade 2/3) was observed in 10% of Hp gerbils, 38% of Hp + salt gerbils, and 29% of Hp + stress gerbils at 52 weeks and 67%, 83%, and 43% at 68 weeks (P < 0.05). Heterotopic proliferative glands were identified in synergy groups from 52 weeks, with increases in their number and size by 68 weeks. Higher proliferative rates were observed in Hp+salt gerbils (P < 0.0001), and p21 overexpression in Hp+salt and Hp+stress gerbils (both P's < 0.0001), by 68 weeks, without p53 increases. We conclude that salt and stress synergize Hp damage and increase pseudo-invasive gland foci. © 2006 Springer Science+Business Media, Inc.
Filiaciones:
Gamboa-Dominguez A.:
Department of Pathology, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Tlalpan, Mexico City, DF 14000, Mexico
Departamento de Patología, INCMNSZ, Mexico City, Mexico
Ubbelohde T.:
Department of Infectious Diseases, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Tlalpan, Mexico City, DF 14000, Mexico
Saqui-Salces M.:
Department of Pathology, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Tlalpan, Mexico City, DF 14000, Mexico
Romano-Mazzoti L.:
Department of Infectious Diseases, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Tlalpan, Mexico City, DF 14000, Mexico
Cervantes M.:
Department of Infectious Diseases, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Tlalpan, Mexico City, DF 14000, Mexico
Domínguez-Fonseca C.:
Department of Pathology, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Tlalpan, Mexico City, DF 14000, Mexico
De La Luz Estreber M.:
Department of Experimental Medicine, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Tlalpan, Mexico City, DF 14000, Mexico
Ruíz-Palacios G.M.:
Department of Infectious Diseases, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Tlalpan, Mexico City, DF 14000, Mexico
Departamento de Infectología, Instituto Nacional de Ciencias Médicas Y Nutrición Salvador Zubirán, Vasco de Quiroga 15, Mexico City, Distrito Federal 14000, Mexico
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