Taenia solium: Identification and preliminary characterization of a lipid binding protein with homology to the SEC14 catalytic domain
Por:
Montero E., Gonzalez L.M., Bonay P., Rosas G., Hernandez B., Sciutto E., Parkhouse R.M.E., Harrison L.J.S., Morales M.A., Garate T.
Publicada:
1 ene 2007
Resumen:
The objective of this work is to identify proteins of the human and porcine parasite, Taenia solium, which may be exploited for control of the parasite. Through screening a cDNA library of T. solium metacestodes, we have identified a novel Sec-14-like Taenia lipid-binding protein that may play an important role in membrane trafficking. The Sec14-like sequence is a single copy gene, encoding a putative polypeptide of 320 amino acids and 36.1 kDa (sec14Tsol protein). Secondary amino acid structural analysis suggested that the sec14Tsol protein might contain two distinct structural domains, an amino-terminal a-helix rich domain and a mixed a-helix/ß-stand carboxy-terminal zone, showing homology with the conserved SEC14 domain found in a great number of proteins that bind lipids, as the regulators of membrane trafficking between Golgi membrane bilayers. Significantly, therefore, in a phosphoinositide-binding assay, sec14Tsol purified recombinant protein specifically interacted with important lipid regulators of membrane trafficking, with a preference for PI(3)P2, PI(3,4)P2, PI(4,5)P2 and phosphatidic acid. Moreover, the sec14Tsol protein was localized in the Golgi apparatus of transfected cells and in the spiral canal region of T. solium metacestode tegument. As sec14Tsol protein may play an important role in membrane trafficking, its demonstrated localisation in the intact parasite tegument suggests its involvement in the function of the tegument and thus perhaps interaction with the host. © 2007 Elsevier Inc. All rights reserved.
Filiaciones:
Montero E.:
Instituto de Salud Carlos III, Centro Nacional de Microbiología, Ctra. Majadahonda Pozuelo Km 2, 28220, Majadahonda Madrid, Spain
Gonzalez L.M.:
Instituto de Salud Carlos III, Centro Nacional de Microbiología, Ctra. Majadahonda Pozuelo Km 2, 28220, Majadahonda Madrid, Spain
Bonay P.:
Centro de Biología Molecular, Universidad Autónoma de Madrid, 28049 Madrid, Spain
Rosas G.:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México DF, Mexico
Hernandez B.:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México DF, Mexico
Sciutto E.:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México DF, Mexico
Parkhouse R.M.E.:
Instituto Gulbenkian de Ciencia, R. Quinta Grande 6, Apartado 14, P-2780-156 Oeiras Codex, Portugal
Harrison L.J.S.:
University of Edinburgh, Royal (Dick) School of Veterinary Studies, Division of Veterinary Clinical Sciences, Easter Bush, Roslin, Midlothian,Scotland EH25 9RG, United Kingdom
Morales M.A.:
Instituto de Salud Carlos III, Centro Nacional de Microbiología, Ctra. Majadahonda Pozuelo Km 2, 28220, Majadahonda Madrid, Spain
Garate T.:
Instituto de Salud Carlos III, Centro Nacional de Microbiología, Ctra. Majadahonda Pozuelo Km 2, 28220, Majadahonda Madrid, Spain
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