The nitrogen assimilation control (Nac) protein represses asnC and asnA transcription in Escherichia coli
Por:
Poggio S., Domeinzain C., Osorio A., Camarena L.
Publicada:
1 ene 2002
Resumen:
In this work, we show that the expression of the asnA and asnC genes is regulated by the availability of ammonium in the growth medium. Our results suggest that, under nitrogen-limiting growth conditions, the nitrogen assimilation control (Nac) protein is involved in the repression of the asnC gene, whose product is required to activate the transcription of asnA. We also show that asparagine negatively affects the expression of asnA, independently of the presence of Nac. These results allow us to conclude that asnA transcription is regulated by two different mechanisms that respond to different effectors: nitrogen and asparagine availability. © 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.
Filiaciones:
Poggio S.:
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ap. Postal 70-228, 04510 México, D.F., Mexico
Domeinzain C.:
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ap. Postal 70-228, 04510 México, D.F., Mexico
Osorio A.:
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ap. Postal 70-228, 04510 México, D.F., Mexico
Camarena L.:
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ap. Postal 70-228, 04510 México, D.F., Mexico
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