Differential expression of a 70 kDa O-glycoprotein on T cells: A possible marker for naive and early activated murine T cells
Por:
Ortíz B., Porras F., Jiménez-Martínez M.C., Montaño L.F., Martínez-Cairo S., Lascurain R., Zenteno E.
Publicada:
1 ene 2002
Resumen:
We purified a 70 kDa O-glycoprotein that binds to the GalNAc specific lectin from Amaranthus leucocarpus (ALLr) and determined its expression pattern on T lymphocytes from different murine lymphoid organs. High level of ALLr expression was demonstrated in 95-98% of both CD4+8+ and CD4-8+ thymocytes, and in 80-95% of CD8+ T cells from peripheral blood, lymph nodes, and spleen, whereas a minor fraction of CD4+8- thymocytes (46-67%) and peripheral CD4+ T cells (9-40%) showed low ALLr expression. Peripheral CD19+ B cells were ALLr negative and most of the peripheral ALL+ T cells showed a CD62LhiCD45RBhiCD44lo/- phenotype, indicating features of naive cells. Mitogenic activation of peripheral T cells increased 3-fold the number of ALL+CD4+ T cells 24 h after stimulation, as opposed to a >80% decrease in CD8+ T cells 72 h after stimulation. Our results suggest that ALL detects a non-described surface O-glycoprotein selectively expressed by naive CD8+ T cells and by early activated CD4+ T cells. © 2002 Elsevier Science (USA). All rights reserved.
Filiaciones:
Ortíz B.:
Departamento de Bioquímica, Inst. Nac. de Enferm. Respiratorias, Calzada de Tlalpan 4502, 14080 Mexico City, Mexico
Porras F.:
Departamento de Bioquímica, Inst. Nac. de Enferm. Respiratorias, Calzada de Tlalpan 4502, 14080 Mexico City, Mexico
Jiménez-Martínez M.C.:
Departamento de Bioquímica, Inst. Nac. de Enferm. Respiratorias, Calzada de Tlalpan 4502, 14080 Mexico City, Mexico
Montaño L.F.:
Departamento de Biologia Celular, Instituto Nacional de Cardiologia, 14080 Mexico City, Mexico
Martínez-Cairo S.:
Unidad de Investigación, Centro Médico Nacional S-XXI, IMSS, 01020 Mexico City, Mexico
Lascurain R.:
Departamento de Bioquímica, Facultad de Medicina, UNAM, 04510 Mexico City, Mexico
Zenteno E.:
Departamento de Bioquímica, Facultad de Medicina, UNAM, 04510 Mexico City, Mexico
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