Lack of correlation between growth inhibition by TGF-ß and the percentage of cells expressing type II TGF-ß receptor in human non-small cell lung carcinoma cell lines
Por:
López-González J.S., Aguilar-Cázares D., Prado-García H., Nieto-Rodríguez A., Mandoki J.J., Avila-Moreno F., Rivera R.M., Chavarría-Garcés J.
Publicada:
1 ene 2002
Resumen:
To determine the mechanisms involved in the evasion from TGF-? growth regulation in the small cell lung carcinoma (SCLC) cell lines and the non-small cell lung carcinoma (NSCLC) cell lines, we studied: (a) production of TGF-?1 and TGF-?2; (b) percentage of cells expressing TGF-? RII; (c) responsiveness of the tumour cell lines to exogenous TGF-?1 or TGF-?2; and (d) presence of mRNA transcripts of the three TGF-? isoforms and of the TGF-? RII. Our results indicate that the SCLC cell lines do not synthesize the isoforms TGF-?1 and TGF-?2 nor the TGF-? RII, thus avoiding inhibitory autocrine and paracrine TGF-? actions. However, NSCLC cell lines express not only TGF-?1, TGF-?2 and TGF-? RII mRNA transcripts, but also synthesize both isoforms and the TGF-? RII. Although approximately 50% of the cells from the studied cell lines expressed the TGF-? RII, different cell lines varied greatly in the sensitivity to the inhibitory action of TGF-?. This could result from alterations in: (i) the structure of TGF-? RII; (ii) the phosphorylation motif of TGF-? RI; (iii) the molecules involved in the intracellular signalling pathway of TGF-?; and (iv) cell cycle regulation. Copyright © 2002 Elsevier Science Ireland Ltd.
Filiaciones:
López-González J.S.:
Depto. Enfermedades Cronico, Inst. Nacional, Enfermedades Respiratorias, Col. Seccion XVI, Mexico D.F. 14080, Mexico
Aguilar-Cázares D.:
Depto. Enfermedades Cronico, Inst. Nacional, Enfermedades Respiratorias, Col. Seccion XVI, Mexico D.F. 14080, Mexico
Prado-García H.:
Depto. Enfermedades Cronico, Inst. Nacional, Enfermedades Respiratorias, Col. Seccion XVI, Mexico D.F. 14080, Mexico
Nieto-Rodríguez A.:
Depto. Enfermedades Cronico, Inst. Nacional, Enfermedades Respiratorias, Col. Seccion XVI, Mexico D.F. 14080, Mexico
Mandoki J.J.:
Departamento de Farmacologia, Facultad de Medicina, Universidad Nacional Autónoma de Mexico, Mexico D.F. 04510, Mexico
Avila-Moreno F.:
Depto. Enfermedades Cronico, Inst. Nacional, Enfermedades Respiratorias, Col. Seccion XVI, Mexico D.F. 14080, Mexico
Rivera R.M.:
Departamento de Patologia, Instituto Nacional de Enfermedades Respiratorias, Tlalpan 4502, Col. Seccion XVI, Mexico D.F. 14080, Mexico
Chavarría-Garcés J.:
Servicio Clinico 3, Instituto Nacional de Enfermedades Respiratorias, Tlalpan 4502, Col. Seccion XVI, Mexico D.F. 14080, Mexico
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