PCR tools for the differential diagnosis of Taenia saginata and Taenia solium taeniasis/cysticercosis from different geographical locations
Por:
Miguel González L., Montero E., Puente S., López-Velez R., Hernández M., Sciutto E., Harrison L.J.S., Parkhouse R.MichaelE., Gárate T.
Publicada:
1 ene 2002
Resumen:
The potential value of PCRs in the species-specific diagnosis of Taenia saginata and Taenia solium have been investigated, using samples of T. saginata and T. solium from different geographical areas. The PCRs examining inter-species differences were based on the sequence of the HDP2 DNA fragment, specific for T. saginata/T. solium, and the sequence of the rDNA internal transcribed spacer 1 and spacer 2 (ITS-1 and ITS-2). This PCR analysis of DNA isolates confirmed morphologic diagnosis and allowed the speciation of samples too small or fragmented for morphologic identification, with clear and consistent inter-species differences between T. saginata (twenty-two) and T. solium (three) geographical isolates. Possible intra-species genomic variability, within these species, was similarly studied through analysis of PCR amplification products (PCR-RFLP) and only encountered one exceptional T. saginata isolate from Kenya, which yielded a unique PCR-RFLP pattern, different from T. saginata DNA of Mexican (one sample) and Spanish (seven samples) origin. © 2002 Elsevier Science Inc. All rights reserved.
Filiaciones:
Miguel González L.:
Instituto de Salud Carlos III, Centro Nacional de Microbiología, Majadahonda, Madrid, Spain
Montero E.:
Instituto de Salud Carlos III, Centro Nacional de Microbiología, Majadahonda, Madrid, Spain
Puente S.:
Instituto de Salud Carlos III, Centro de Investigaciones Cli´nicas, Madrid, Spain
López-Velez R.:
Hospital Ramón y Cajal, Madrid, Spain
Hernández M.:
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México D.F., México, Mexico
Sciutto E.:
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México D.F., México, Mexico
Harrison L.J.S.:
University of Edinburgh, Department of Tropical Animal Health, Sir Alexander Robertson Centre for Tropical Veterinary Medicine, United Kingdom
Gárate T.:
Instituto de Salud Carlos III, Centro Nacional de Microbiología, Majadahonda, Madrid, Spain
Gulbenkian Institute for Science, R. Quinta Grande 6, Apartado 14, P-2781 Oeiras Codex, Portugal
|