Characterization of extracellular polymers synthesized by tropical intertidal biofilm bacteria
Por:
Ortega-Morales B.O., Santiago-García J.L., Chan-Bacab M.J., Moppert X., Miranda-Tello E., Fardeau M.L., Carrero J.C., Bartolo-Pérez P., Valadéz-González A., Guezennec J.
Publicada:
1 ene 2007
Resumen:
Aim: This study was performed to determine the potential of tropical intertidal biofilm bacteria as a source of novel exopolymers (EPS). Methods and Results: A screening procedure was implemented to detect EPS-producing biofilm bacteria. Isolates MC3B-10 and MC6B-22, identified respectively as a Microbacterium species and Bacillus species by 16S rDNA and cellular fatty acids analyses, produced different EPS, as evidenced by colorimetric and gas chromatographic analyses. The polymer produced by isolate MC3B-10 displays significant surfactant activity, and may chelate calcium as evidenced by spectroscopic analysis. Conclusions: Polymer MC3B-10 appears to be a glycoprotein, while EPS MC6B-22 seems to be a true polysaccharide dominated by neutral sugars but with significant concentrations of uronic acids and hexosamines. EPS MC3B-10 possesses a higher surfactant activity than that of commercial surfactants, and given its anionic nature, may chelate cations thus proving useful in bioremediation. The chemical composition of polymer MC6B-22 suggests its potential biomedical application in tissue regeneration. Significance and Impact of the Study: This is the first report of a Microbacterium species producing EPS with surfactant properties, which expands our knowledge of the micro-organisms capable of producing these biomolecules. Furthermore, this work shows that tropical intertidal environments are a nonpreviously recognized habitat for bioprospecting EPS-producing bacteria, and that these molecules might be involved in ecological roles protecting the cells against dessication. © 2007 The Authors.
Filiaciones:
Ortega-Morales B.O.:
Departamento de Microbiología Ambiental Y Biotecnología, Programa de Corrosión del Golfo de México, Universidad Autónoma de Campeche Av., Campeche, Mexico
Departamento de Microbiología Ambiental Y Biotecnología, Programa de Corrosión del Golfo de México, Universidad Autónoma de Campeche Av., Agustin Melgar s/n, Col. Buenavista, C.P. 24030, Campeche, Mexico
Santiago-García J.L.:
Departamento de Microbiología Ambiental Y Biotecnología, Programa de Corrosión del Golfo de México, Universidad Autónoma de Campeche Av., Campeche, Mexico
Chan-Bacab M.J.:
Departamento de Microbiología Ambiental Y Biotecnología, Programa de Corrosión del Golfo de México, Universidad Autónoma de Campeche Av., Campeche, Mexico
Moppert X.:
Ifremer, Plouzane, France
Miranda-Tello E.:
Institute de Recherche Pour Le Développement UR-101, Extrêmophiles, Universités de Provence et de la Méditerranée, Marseille, Cedex 09, France
Fardeau M.L.:
Institute de Recherche Pour Le Développement UR-101, Extrêmophiles, Universités de Provence et de la Méditerranée, Marseille, Cedex 09, France
Carrero J.C.:
Department of Immunology, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, D.F., Mexico
Bartolo-Pérez P.:
Departamento de Microbiología Ambiental Y Biotecnología, Programa de Corrosión del Golfo de México, Universidad Autónoma de Campeche Av., Campeche, Mexico
CINVESTAV-IPN Mérida, Departamento de Física-Aplicada, Yucatán, Mexico
Valadéz-González A.:
Departamento de Materiales, Centro de Investigación Científica de Yucatán, Yucatán, Mexico
Guezennec J.:
Ifremer, Plouzane, France
All Open Access; Green
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