Potential regulatory elements in the Trypanosoma cruzi rRNA gene promoter
Por:
Figueroa-Angulo E., María Cevallos A., Zentella A., López-Villaseñor I., Hernández R.
Publicada:
1 ene 2006
Resumen:
The Trypanosoma cruzi rRNA gene promoter was characterized by deletion and point mutation analyses. A core of 89 bp was identified as the minimal region with full promoter activity. This core region is flanked upstream by a control element that stimulates its activity, and downstream by a novel down regulating region of about 200 bp. A point mutation analysis of the transcription start region evidenced 7 contiguous nucleotides where individual substitutions produced in all cases a defective promoter. It is generally accepted that the anciently speciated trypanosomatids lack strict promoters for protein coding genes transcribed by RNA polymerase II. The occurrence of a well structured rRNA gene promoter in these species suggests an early appearance of the RNA polymerase I promoters in the evolution of eukaryotic cells. © 2006 Elsevier B.V. All rights reserved.
Filiaciones:
Figueroa-Angulo E.:
Departamento de Biología Molecular Y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado Postal 70-228, 04510 México D.F., Mexico
María Cevallos A.:
Departamento de Biología Molecular Y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado Postal 70-228, 04510 México D.F., Mexico
Zentella A.:
Departamento de Biología Molecular Y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado Postal 70-228, 04510 México D.F., Mexico
Departamento de Bioquímica, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Secretaría de Salud, CP14000 México D.F., Mexico
López-Villaseñor I.:
Departamento de Biología Molecular Y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado Postal 70-228, 04510 México D.F., Mexico
Hernández R.:
Departamento de Biología Molecular Y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado Postal 70-228, 04510 México D.F., Mexico
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