Differential molecular identification of Taeniid spp. and Sarcocystis spp. cysts isolated from infected pigs and cattle
Por:
González L.M., Villalobos N., Montero E., Morales J., Sanz R.A., Muro A., Harrison L.J.S., Parkhouse R.M.E., Gárate T.
Publicada:
1 ene 2006
Resumen:
In the present work, the species-specific identification of Taeniid spp. cysticerci and sarcocystis cysts isolated from infected pigs and cattle was achieved by PCR. In particular: (i) multiplex-PCR derived from HDP2 DNA fragment, specific for Taenia saginata/Taenia solium; (ii) PCRs and PCR-RFLPs of the rDNA internal transcribed spacers 1 and 2 (ITS1 and ITS2) for the differential diagnosis of taeniids; (iii) PCR derived from the 18S rRNA gene and sequencing, specific for Sarcoystis spp. The combined application of these three PCR protocols provided an unequivocally specific diagnosis of T. saginata, T. solium, T. hydatigena, Sarcocystis hominis and Sarcocystis suihominis, and may have practical application in the identification of calcified degenerating or morphologically dubious cysts, for example in the slaughter house situation or in human biopsy samples. © 2006 Elsevier B.V. All rights reserved.
Filiaciones:
González L.M.:
Servicio de Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain
Villalobos N.:
Servicio de Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain
Montero E.:
Servicio de Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain
Morales J.:
Facultad de Medicina Veterinaria y Zootecnia, la Universidad Nacional Autónoma de México, México 04510, Mexico
Sanz R.A.:
Dirección General de Salud Pública y Asistencia, Consejería de Sanidad, Junta de Castilla y León, 47071 Valladolid, Spain
Muro A.:
Departamento de Parasitología, Facultad de Farmacia, Universidad de Salamanca, Salamanca, Spain
Harrison L.J.S.:
University of Edinburgh, Royal (Dick) School of Veterinary Studies, Division of Veterinary Clinical Sciences (incorporating Centre for Tropical Veterinary Medicine), Easter Bush, Roslin, Midlothian Scotland EH25 9RG, United Kingdom
Parkhouse R.M.E.:
Instituto Gulbenkian de Ciencia, R. Quinta Grande 6, Apartado 14, P-2781 Oeiras Codex, Portugal
Gárate T.:
Servicio de Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain
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