Toluidine blue-O staining of prion protein deposits
Por:
Sánchez A., Guzmán A., Ortiz A., Rembao D., Espinosa B., Zenteno E., Guevara J.
Publicada:
1 ene 2001
Resumen:
Prion diseases or transmissible spongiform encephalopathies are a group of fatal neurodegenerative diseases caused by an abnormal form of prion protein (PrP sc). In this study, we developed a sensitive histo-chemical detection of PrPsc deposits in a Gertsmann-Sträussler-Scheinker disease (GSS) patient using tolui-dine blue-O staining, a specific reagent to stain mucins and mucopolysaccharides. Detection of prion deposits correlated with immunohistochemistry using anti-prion antibodies. Control assays were performed using amyloid-?(A?) plaques from Alzheimer's disease (AD) brains. Our results demonstrated that toluidine blue-O staining allowed to recognize 69.1±2.6% of the total plaques recognized by the anti-prion antibody. Furthermore, in the 15 studied brain regions from the GSS patient, toluidine blue-O revealed the same recognition pattern as anti-prion labeling. Toluidine blue-O stained specifically the prion deposits but not the A? plaques in AD brains. The specificity of the technique was confirmed in a Creutzfeldt-Jakob disease brain. This method opens several possibilities for postmortem diagnoses. Our results also suggest the relevance of specific post-translational modifications of PrP sc, identified by toluidine blue-O, that might participate in the transformation of PrP c to PrP sc.
Filiaciones:
Sánchez A.:
Departamento de Neuropatología, Instituto Nacional de Neurología, Insurgentes Sur 3877, La Fama, 14269 Mexico, DF, Mexico
Guzmán A.:
Departamento de Neuroanatomía, Instituto Nacional de Neurología, 14269 Mexico, DF, Mexico
Ortiz A.:
Departamento de Neuropatología, Instituto Nacional de Neurología, Insurgentes Sur 3877, La Fama, 14269 Mexico, DF, Mexico
Rembao D.:
Departamento de Neuropatología, Instituto Nacional de Neurología, Insurgentes Sur 3877, La Fama, 14269 Mexico, DF, Mexico
Espinosa B.:
Departamento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, 10245 Mexico, DF, Mexico
Zenteno E.:
Departamento de Bioquímica, Facultad de Medicina, UNAM, 04510 Mexico, DF, Mexico
Guevara J.:
Departamento de Neuropatología, Instituto Nacional de Neurología, Insurgentes Sur 3877, La Fama, 14269 Mexico, DF, Mexico
|