High-risk human papilloma virus infection decreases the frequency of dendritic Langerhans' cells in the human female genital tract
Por:
Jimenez-Flores R., Mendez-Cruz R., Ojeda-Ortiz J., Muñoz-Molina R., Balderas-Carrillo O., De La Luz Diaz-Soberanes M., Lebecque S., Saeland S., Daneri-Navarro A., Garcia-Carranca A., Ullrich S.E., Flores-Romo L.
Publicada:
1 ene 2006
Resumen:
Dendritic cells (DC) are often arranged in planar layers in tissues with high antigenic exposure, such as skin and mucosae. Providing an en face view, this arrangement optimizes in situ analysis regarding morphology (even of individual dendrites), topographic distribution (regular/clustered) and quantification. The few reports on human genital DC usually utilize single markers and conventional sections, restricting immunolabelling only to cell parts sectioned by the cut. To better assess DC in situ, we labelled epithelial sheets, prepared from fresh cervix biopsies, with antibodies to major histocompatibility complex (MHC)-CII, CD1a and Langerin, revealing (with each of these markers) a dense DC network in a planar-like, regular distribution. Using the hybrid capture system to detect the high-risk mucotropic human papilloma virus (HPV) group, 16 positive and five negative women were studied and the results were compared between these groups. DC frequency per area was substantially reduced (to ?50% for the three markers) in samples from all HPV-infected patients compared with samples from controls. Unlike HPV - samples, Langerin+ DC in HPV+ cervix exhibited a highly accentuated dendritic appearance. We believe this to be the first study using these three DC-restricted markers (Langerin, CD1a and MHC-CII) in cervical epithelial sheets from high-risk HPV+ donors and also the first study to demonstrate the morphological and quantitative changes triggered by high-risk HPV infection. Cervical DC reduction in early, premalignant high-risk HPV infection might represent viral subversion strategies interfering with efficient antigen handling by the immune system's peripheral sentinels, the DC, perhaps hampering appropriate recruitment and subsequent development of effector (cytotoxic) T cells. © 2005 Blackwell Publishing Ltd.
Filiaciones:
Jimenez-Flores R.:
Department of Experimental Pathology, CINVESTAV-IPN, Mexico, Mexico
Faculty of High Studies, Iztacala, UNAM, Mexico, Mexico
Mendez-Cruz R.:
Department of Experimental Pathology, CINVESTAV-IPN, Mexico, Mexico
Faculty of High Studies, Iztacala, UNAM, Mexico, Mexico
Ojeda-Ortiz J.:
Cervical Pathology and Colposcopy Unit, Pachuca Regional Hospital, Mexico, Mexico
Muñoz-Molina R.:
Cervical Pathology and Colposcopy Unit, Pachuca Regional Hospital, Mexico, Mexico
Balderas-Carrillo O.:
Department of Cell Biology, CINVESTAV-IPN, Mexico, Mexico
De La Luz Diaz-Soberanes M.:
Department of Cell Biology, CINVESTAV-IPN, Mexico, Mexico
Gyneco-Obstetrics Hospital, IMSS, Tlatelolco, Mexico
Lebecque S.:
Laboratory for Immunological Research, Lyon, France
Saeland S.:
Laboratory for Immunological Research, Lyon, France
Daneri-Navarro A.:
Health Sciences Center, CUCS, University of Guadalajara, Mexico, Mexico
Garcia-Carranca A.:
Biomedical Research Institute, UNAM, Mexico, Mexico
Ullrich S.E.:
Department of Immunology, MD Anderson Cancer Center, Houston, TX, United States
Flores-Romo L.:
Department of Cell Biology, CINVESTAV-IPN, Mexico, Mexico
Department of Cell Biology, CINVESTAVIPN, Avenue IPN 2508, Zacatenco CP 07360, Mexico City, Mexico
All Open Access, Bronze
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