Different antiamebic antibody isotype patterns in the large and small intestine after local and systemic immunization of mice with glutaraldehyde fixed Entamoeba histolytica trophozoites
Por:
Moreno-Fierros L., Reséndiz-Albor A.A., López-Revilla R.
Publicada:
1 ene 1999
Resumen:
We have determined the major immunoglobulin isotypes (IgG, IgA, IgM) of antiamebic antibodies induced in the serum and in the large and small intestine after local (oral and rectal) or systemic (intraperitoneal and intramuscular) immunization of mice with glutaraldehyde-fixed Entamoeba histolytica trophozoites (GFT). IgA predominated in the small intestine after immunization through all routes, whereas in the large intestine similar antibody levels of the major isotypes were induced by rectal, intraperitoneal and intramuscular immunization. The intramuscular route elicited intestinal responses lower than those induced by the rectal and intraperitoneal routes, but higher than the slight IgA antibody increase observed after oral immunization. The differences in antiamebic antibody response patterns at the large and small intestine suggest that there are different mucosal effector compartments. They also indicate that isotype analysis of mucosal antibodies from the sites where an infectious agent resides is needed to evaluate whether a vaccine candidate induces responses of higher protective value in the appropriate site, and that the study of antibody responses must not be limited to sampling the serum or mucosal sites distant to the relevant one.
Filiaciones:
Moreno-Fierros L.:
Unidad de Morfologia y Funcion, ENEP-Iztacala, Universidad Nacional Autonoma Mexico, Ap. postal 314, Tlalnepantla, Méx., Mexico
UMF ENEP Iztacala-UNAM, Av. de los Barrios s/n, Tlalnepantla, Méx., Mexico
Reséndiz-Albor A.A.:
Unidad de Morfologia y Funcion, ENEP-Iztacala, Universidad Nacional Autonoma Mexico, Ap. postal 314, Tlalnepantla, Méx., Mexico
López-Revilla R.:
Departamento de Biologia Celular, Ctro. Invest. Estud. Avanzados IPN, Ap. postal 14-740, 07000 México, D.F., Mexico
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