Molecular characterization of the tight junction protein ZO-1 in MDCK cells
Por:
González-Mariscal L., Islas S., Contreras R.G., García-Villegas M.R., Betanzos A., Vega J., Diaz-Quiñónez A., Martín-Orozco N., Ortiz-Navarrete V., Cereijido M., Valdés J.
Publicada:
1 ene 1999
Resumen:
Most of the information on the structure and function of the tight junction (TJ) has been obtained in MDCK cells. Accordingly, we have sequenced ZO-1 in this cell type, because this protein is involved in the response of the TJ to changes in Ca2+, phosphorylation, and the cytoskeleton. ZO-1 of MDCK cells comprises 6805 bp with a predicted open reading frame of 1769 amino acids. This sequence is 92 and 87% homologous to human and mouse ZO-1, respectively. Two nuclear sorting signals located at the PDZ1 and GK domains and 17 SH3 putative binding sites at the proline-rich domain were detected. We found two new splicing regions at the proline-rich region: ? had not been reported in human and mouse counterparts, and ?, which was previously sequenced in human and mouse ZO-1, is now identified as a splicing region. The expression of different ? and ? isoforms varies according to the tissue tested. With the information provided by the sequence, Southern blot, and PCR experiments we can predict a single genomic copy of MDCK. ZO-1 that is at least 13.16 kb long. MDCK-ZO-1 mRNA is 7.4 kb long. Its expression is regulated by calcium, while the expression of MDCK-ZO-1 protein is not.
Filiaciones:
González-Mariscal L.:
Department of Physiology, Biophysics, and Neuroscience, Ctr. Res. Adv. Studs. (CINVESTAV), Apdo. Postal 14-740, 07000, México, D.F., Mexico
Islas S.:
Department of Physiology, Biophysics, and Neuroscience, Ctr. Res. Adv. Studs. (CINVESTAV), Apdo. Postal 14-740, 07000, México, D.F., Mexico
Contreras R.G.:
Department of Physiology, Biophysics, and Neuroscience, Ctr. Res. Adv. Studs. (CINVESTAV), Apdo. Postal 14-740, 07000, México, D.F., Mexico
García-Villegas M.R.:
Department of Physiology, Biophysics, and Neuroscience, Ctr. Res. Adv. Studs. (CINVESTAV), Apdo. Postal 14-740, 07000, México, D.F., Mexico
Betanzos A.:
Department of Physiology, Biophysics, and Neuroscience, Ctr. Res. Adv. Studs. (CINVESTAV), Apdo. Postal 14-740, 07000, México, D.F., Mexico
Vega J.:
Dept. of Genet. and Molec. Biology, Ctr. Res. Adv. Studs. (CINVESTAV), Apdo. Postal 14-740, 07000, México, D.F., Mexico
Diaz-Quiñónez A.:
U. Invest. Medica en Inmunoquimica, Ctro. Médico Nac. Siglo XX1, IMSS, México, D.F., Mexico
Martín-Orozco N.:
U. Invest. Medica en Inmunoquimica, Ctro. Médico Nac. Siglo XX1, IMSS, México, D.F., Mexico
Ortiz-Navarrete V.:
U. Invest. Medica en Inmunoquimica, Ctro. Médico Nac. Siglo XX1, IMSS, México, D.F., Mexico
Cereijido M.:
Department of Physiology, Biophysics, and Neuroscience, Ctr. Res. Adv. Studs. (CINVESTAV), Apdo. Postal 14-740, 07000, México, D.F., Mexico
Valdés J.:
Department of Physiology, Biophysics, and Neuroscience, Ctr. Res. Adv. Studs. (CINVESTAV), Apdo. Postal 14-740, 07000, México, D.F., Mexico
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