Hepatocyte production of modulators of extracellular liver matrix in normal and cirrhotic rat liver
Por:
Del Carmen Garcíade León M., Montfort I., Tello Montes E., López Vancell R., Olivos García A., González Canto A., Nequiz-Avendaño M., Pérez-Tamayo R.
Publicada:
1 ene 2006
Resumen:
In the present study, we found collagenolytic and gelatinolytic activity in the supernatants of hepatocyte cultures from rats with experimental CCl 4-induced liver cirrhosis, in levels significantly higher than in comparable supernatants of hepatocyte cultures from normal rats. In addition, we clearly detected the messenger ribonucleic acids (mRNA) of four matrix metalloproteinases (MMP-2, MMP-3, MMP-10, and MMP-13) and of two tissue inhibitors of matrix metalloproteinases (TIMP-1 and TIMP-2) in hepatocytes from both normal and cirrhotic rats by RT-PCR and by in situ hybridization. Finally, we demonstrated MMP-2, MMP-3, and MMP-13 and TIMP-1 and TIMP-2 proteins in the same hepatocyte preparations by immunostaining. We conclude that rat hepatocytes produce the major enzymes and inhibitors involved in liver ECM modulation and therefore suggests that they might participate actively in the pathophysiology of liver cirrhosis in rats. © 2005 Elsevier Inc. All rights reserved.
Filiaciones:
Del Carmen Garcíade León M.:
Departamento de Medicina Experimental, Facultad de Medicina, Hospital General, De México, D.F. 06720, Mexico
Montfort I.:
Departamento de Medicina Experimental, Facultad de Medicina, Hospital General, De México, D.F. 06720, Mexico
Tello Montes E.:
Departamento de Medicina Experimental, Facultad de Medicina, Hospital General, De México, D.F. 06720, Mexico
López Vancell R.:
Departamento de Medicina Experimental, Facultad de Medicina, Hospital General, De México, D.F. 06720, Mexico
Olivos García A.:
Departamento de Medicina Experimental, Facultad de Medicina, Hospital General, De México, D.F. 06720, Mexico
González Canto A.:
Departamento de Medicina Experimental, Facultad de Medicina, Hospital General, De México, D.F. 06720, Mexico
Nequiz-Avendaño M.:
Departamento de Medicina Experimental, Facultad de Medicina, Hospital General, De México, D.F. 06720, Mexico
Pérez-Tamayo R.:
Departamento de Medicina Experimental, Facultad de Medicina, Hospital General, De México, D.F. 06720, Mexico
Departamento de Medicina Experimental, Facultad de Medicina, UNAM, Apartado Postal 70-641, D.F. 04510 México, Mexico
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