Nitric oxide/cGMP mediates the spasmolytic action of 3,4'-dihydroxy- 5,5'-dimethoxybibenzyl from Scaphyglottis livida
Por:
Estrada S., Rojas A., Mathison Y., Israel A., Mata R.
Publicada:
1 ene 1999
Resumen:
Five aromatic compounds, 3,4'-dihydroxy-5,5'-dimethoxybibenzyl (1), batatasin III (2), coelonin (3), 3,7-dihydroxy-2,4-dimethoxyphenanthrene (4), and 3,7-dihydroxy-2,4,8-trimethoxyphenanthrene (5) were isolated from the orchid Scaphyglottis livida (Lindley) Schltr. Compounds 1-5 induced a concentration-dependent inhibition of the spontaneous contractions of the rat ileum with potencies comparable or higher to that of papaverine. The relaxation evoked by compounds 14 was blocked by L-NAME, an inhibitor of nitric oxide synthase. It was also demonstrated that 1 increased cyclic GMP content in rat ileum rings. Compound 1-induced elevation of cGMP was inhibited by L-NAME and ODQ, inhibitors of nitric oxide synthase and soluble guanylyl cyclase, respectively. These results indicate that nitric oxide/cGMP formation constitute the signaling pathway in the spasmolytic action of compound 1.
Filiaciones:
Estrada S.:
Departamento de Farmacia, Facultad de Química, Universidad Nac. Autonoma de Mexico, Coyoacan, 04510 Mexico City, Mexico
Rojas A.:
Facultad de Química, Universidad Autónoma de Querétaro, Querétaro, Mexico
Mathison Y.:
Fac. de Medicina Jose Maria Vargas, Universidad Central de Venezuela, Caracas, Venezuela
Facultad de Farmacia, Universidad Central de Venezuela, Caracas, Venezuela
Israel A.:
Facultad de Farmacia, Universidad Central de Venezuela, Caracas, Venezuela
Mata R.:
Departamento de Farmacia, Facultad de Química, Universidad Nac. Autonoma de Mexico, Coyoacan, 04510 Mexico City, Mexico
|