Intestinal amoebiasis: antibody-secreting cells and humoral antibodies
Por:
Ghosh P.K., Gupta S., Leon L.R., Ghosh R., Ruiz Ordaz B.H., Ortiz-Ortiz L.
Publicada:
1 ene 1998
Resumen:
Splenic plasma cell response and systemic antibody response to intestinal amoebiasis were studied in C 3H/HeJ mice from 5 to 60 days post-inoculation with Entamoeba histolytica. At various time intervals specific antibody-secreting cells (ASC) in the spleen were measured in infected mice and non-infected control mice by enzyme-linked immunospot (ELISPOT) assay. Serum antibodies were measured by enzyme-linked immunosorbent assay (ELISA). The infected animals showed high IgA ASC from 30 to 50 days postinoculation as compared to IgM and IgG ASC. However, class-specific serum antibody showed high IgG titre from 30 to 60 days post-inoculation as compared to IgM and IgA serum titres. Our results suggest that E. histolytica trophozoites can induce a plasma cell response in the spleen that is different from anti-amoebic antibody response in serum. © 1998 International Centre for Dianhoeal Disease Research,.
Filiaciones:
Ghosh P.K.:
Departments of Immunology, India
Department of Immunology, Kothari Medical Centre and Research Institute, 8/3 Alipore Road, Calcutta 700 027, India
Gupta S.:
Departments of Immunology, India
Leon L.R.:
Department of Molecular Biology, Universidad National Autonoma de Mexico. D.F, Mexico
Ghosh R.:
Departments of Immunology, India
Ruiz Ordaz B.H.:
Department of Molecular Biology, Universidad National Autonoma de Mexico. D.F, Mexico
Ortiz-Ortiz L.:
Departments of Immunology, India
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