Inhibition of phagocytic activity by the N-acetyl-D-galactosamine-specific lectin from Amaranthus leucocarpus
Por:
Maldonado G., Gorocica P., Agundis C., Pérez A., Molina J., Zenteno E.
Publicada:
1 ene 1998
Resumen:
Amaranthus leucocarpus lectin (ALL), specific for N-acetyl-D-galactosamine, induces inhibition of the erythrophagocytic activity of resident murine peritoneal macrophages and of the macrophage-like cell line J-774. This effect was observed only in macrophages that were Mac-2 (CD11c/CD18 or CR4) negative, indicating that macrophage activation induces important modification to the glycosylation (mainly O-glycosylation) of the membrane. Receptors for IgM and C3b remain unaltered after lectin treatment. Ultrastructural analysis revealed (a) that ALL induced the formation of pinocytic vacuoles, and (b) a regular distribution over the macrophage membrane as well as endosomal vesicles of the gold labeled ALL. Our results suggest that macrophage membrane glycoproteins with constitutive N-acetyl-D-galactosamine residues participate in the regulation of pinocytic-phagocytic vacuole formation.
Filiaciones:
Maldonado G.:
Laboratorio de Inmunología, Departamento de Bioquímica, Facultad de Medicina UNAM, P.O. BOX 70159, 04510 México, Mexico
Gorocica P.:
Departmento de Bioquímica, Inst. Nac. de Enferm. Respiratorias, Tlalpan, 14080 México, Mexico
Agundis C.:
Laboratorio de Inmunología, Departamento de Bioquímica, Facultad de Medicina UNAM, P.O. BOX 70159, 04510 México, Mexico
Pérez A.:
Departmento de Biol. Cel. y Tisular, Facultad de Medicina UNAM, 04510 México, Mexico
Molina J.:
Departmento de Farmacología, Facultad de Medicina UNAM, 04510 México, Mexico
Zenteno E.:
Laboratorio de Inmunología, Departamento de Bioquímica, Facultad de Medicina UNAM, P.O. BOX 70159, 04510 México, Mexico
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