Molecular and functional characterization and tissue localization of 2 glucose transporter homologues (TGTP1 and TGTP2) from the tapeworm Taenia solium
Por:
Rodríguez-Contreras D., Skelly P.J., Landa A., Shoemaker C.B., Laclette J.P.
Publicada:
1 ene 1998
Resumen:
Tapeworms absorb and consume large quantities of glucose through their syncytial tegument, storing the excess as glycogen. Although some studies on the metabolism of glucose in several tapeworms are available, the proteins that mediate its uptake and distribution in their tissue have not been identified. We describe the isolation and characterization of cDNA clones encoding 2 facilitated diffusion glucose transporters (TGTP1 and TGTP2) from Taenia solium, the causal agent of human and porcine cysticercosis. Radio-isotope labelled hexose uptake mediated by TGTP1 expressed in Xenopus oocytes is inhibited by the natural stereoisomers D-glucose and D-mannose but not by L-glucose. Transport by TGTP1 is sensitive to classical inhibitors of facilitated diffusion such as phloretin and cytochalasin B, and insensitive to ouabain. TGTP2 did not function in Xenopus oocytes. Localization studies using specific anti-TGTP1 and anti-TGTP2 antibodies show that TGTP1 is abundant in a number of structures underlying the tegument in adult parasites and larvae, whereas TGTP2 appears to be localized only on the tegumentary surface of the larvae and is not detected in adults.
Filiaciones:
Rodríguez-Contreras D.:
Department of Immunology, Inst. de Invest. Biomédicas, Univ. Nac. Auton. de México, A.P. 70228, 04510 México D.F., Mexico
Skelly P.J.:
Dept. of Immunol. and Infect. Dis., Harvard School of Public Health, 665 Huntington Ave., Boston, MA 02115, United States
Landa A.:
Dept. of Microbiol. and Parasitology, Facultad de Medicina, Univ. Nac. Auton. de México, A.P. 70228, 04510 México D.F., Mexico
Shoemaker C.B.:
Dept. of Immunol. and Infect. Dis., Harvard School of Public Health, 665 Huntington Ave., Boston, MA 02115, United States
Laclette J.P.:
Department of Immunology, Inst. de Invest. Biomédicas, Univ. Nac. Auton. de México, A.P. 70228, 04510 México D.F., Mexico
Department of Immunology, Inst. de Invest. Biomédicas, UNAM, A.P. 70228, 04510 México D.F., Mexico
|