Correlation between the kinetics of Th1/Th2 cells and pathology in a murine model of experimental pulmonary tuberculosis
Por:
Hernández-Pando R., Orozcoe H., Sampieri A., Pavón L., Velasquillo C., Larriva-Sahd J., Alcocer J.M., Madrid M.V.
Publicada:
1 ene 1996
Resumen:
T-helper 1 (Th1)/Th2 kinetics were studied by immunohistochemistry and molecular biology techniques (reverse transcriptase-polymerase chain reaction: RT-PCR, Southern-blot) during the course of pulmonary tuberculosis induced in BALB/c mice by the intratracheal instillation of the live and virulent strain H-37Rv. The histopathological study clearly showed two phases of the disease. The first one was an acute phase which was characterized by inflammatory infiltrate in the alveolar-capillary interstitium, blood vessel and bronchial wall with formation of granulomas. In this acute phase, which lasted from 1 to 28 days, a clear predominance of Th1 cells was observed, manifested by a high percentage of interleukin-2 (IL-2) positive cells in the inflammatory infiltrate and granulomas demonstrated by immunohistology, as well as a gradual increment of interferon-? (INF-?) m-RNA. This was followed by a chronic or advanced phase characterized by pneumonia, focal necrosis and fibrosis, with a Th0 balance due to an equivalent proportion of IL-2 and IL-4 positive cells in the lung lesions, that coincided with the highest level of INF-? and IL-4 mRNA. The cytofluorometric analysis of bronchial lavage cells, showed a predominance of CD4 T cells during the acute phase and CD8 T lymphocytes in the chronic phase. ?-? T lymphocytes showed two peaks, at the beginning (3 days) and at the end (4 months) of the infection. These results suggest that T-lymphocyte subset kinetics and the pattern of cytokines produced in the lung during tuberculosis infection changed over time and correlate with the type and magnitude of tissue injury.
Filiaciones:
Hernández-Pando R.:
Department of Pathology, Inst. Nac. Nutr. 'Salvador Zubiran', Mexico City, Mexico
Departamento de Patología, Inst. Nac. Nutr. 'Salvador Zubiran', Calle Vasco de Quiroga 15, Delagacion Tlalpan, 14000, Mexico
Orozcoe H.:
Department of Pathology, Inst. Nac. Nutr. 'Salvador Zubiran', Mexico City, Mexico
Sampieri A.:
Department of Molecular Virology, Ctro. Invest. Sobre Infermedades I., Cuernavaca Mor, Mexico
Pavón L.:
Department of Pathology, Inst. Nac. Nutr. 'Salvador Zubiran', Mexico City, Mexico
Velasquillo C.:
Department of Immunology, Inst. Nac. Nutr. 'Salvador Zubiran', Mexico City, Mexico
Larriva-Sahd J.:
Department of Pathology, Inst. Nac. Nutr. 'Salvador Zubiran', Mexico City, Mexico
Facultad Mexicana de Medicina, Universidad La Salle, México, Mexico
Alcocer J.M.:
Department of Molecular Virology, Ctro. Invest. Sobre Infermedades I., Cuernavaca Mor, Mexico
Madrid M.V.:
Department of Molecular Virology, Ctro. Invest. Sobre Infermedades I., Cuernavaca Mor, Mexico
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