Cloning and characterization of an extracellular Ca2+-sensing receptor from bovine parathyroid
Por:
Brown E.M., Gamba G., Riccardi D., Lombardi M., Butters R., Kifor O., Sun A., Hediger M.A., Lytton J., Hebert S.C.
Publicada:
1 ene 1993
Resumen:
Maintenance of a stable internal environment within complex organisms requires specialized cells that sense changes in the extracellular concentration of specific ions (such as Ca2+). Although the molecular nature of such ion sensors is unknown, parathyroid cells possess a cell surface Ca2+-sensing mechanism that also rec-ognizes trivalent and polyvalent cations (such as neomycin) and couples by changes in phosphoinositide turnover and cytosolic Ca2+ to regulation of parathyroid hormone secretion1-4. The latter restores normocalcaemia by acting on kidney and bone2. We now report the cloning of complementary DNA encoding an extracellular Ca2+ -sensing receptor from bovine parathyroid with pharmacological and functional properties nearly identical to those of the native receptor. The novel & sim;120K receptor shares limited similarity with the metabotropic glutamate receptors5 and features a large extracellular domain, containing clusters of acidic aminoacid residues possibly involved in calcium binding, coupled to a seven-membrane-spanning domain like those in the G-protein-coupled receptor superfamily. © 1993 Nature Publishing Group.
Filiaciones:
Endocrine-Hypertension Division, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, United States
Harvard Center for the Study of Kidney Disease, Harvard Medical School, Brigham and Women's Hospital, Boston, MA 02115, United States
Department of Nephrology, Institute Nacional de la Nutricion Salvador Zubiran, Tlalpan 14000, Mexico City, Mexico
Renal Division, Department of Medicine, Brown University, RI 02963, United States
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