Organization of the double-stranded RNA-activated protein kinase DAI and virus-associated VA RNAI in adenovirus-2-infected HeLa cells
Por:
Jimenez-Garcia L.F., Green S.R., Mathews M.B., Spector D.L.
Publicada:
1 ene 1993
Resumen:
We have examined the cellular distribution of the double-stranded RNA-activated protein kinase DAI in adenovirus 2 (Ad2) -infected and uninfected HeLa cells. In uninfected cells DAI was found to be concentrated in the cytoplasm. In addition, DAI was localized in the nucleoli and diffusely distributed throughout the nucleoplasm. Cells treated with ?-interferon displayed a similar pattern of distribution for DAI. When RNA polymerase I activity was inhibited by the drug actinomycin D, nucleoli segregated and DAI was found to colocalize with the dense fibrillar region of the nucleoli. During mitosis, the distribution of DAI paralleled that of rRNA. In adenovirus-infected cells the localization of DAI was similar to that in uninfected interphase cells. VA RNAI was detected in Ad2-infected cells by 10-14 hours postinfection as fine dots in the nucleoplasm. By 18-24 hours post-infection, VA RNAI appeared in bigger and more abundant dots in the nucleoplasm and the cytoplasm was intensively labeled. Transient expression of the VA RNAI gene in uninfected cells resulted in a similar localization of the RNA. Our results are consistent with a role for DAI and VA RNAI in protein synthesis and suggest that DAI may play an early role in ribosome biogenesis in the nucleolus in addition to its cytoplasmic role in translation.
Filiaciones:
Jimenez-Garcia L.F.:
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, United States
Green S.R.:
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, United States
Mathews M.B.:
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, United States
Spector D.L.:
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, United States
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