Non-pathogenic Entamoeba histolytica: functional and biochemical characterization of a monoxenic strain
Por:
Vargas M.A., Isibasi A., Kumate J., Orozco E.
Publicada:
1 ene 1990
Resumen:
We have cultured under monoxenic conditions and characterized an Entamoeba histolytica clone, MAV-I CINVESTAV (MAV-I), obtained from feces from an asymptomatic carrier. The clone shows the non-pathogenic E. histolytica zymodeme type I, which did not change through the process of monoxenization. Clone MAV-I was non-pathogenic in both in vivo and in vitro tests, and it did not have a functional 112-kDa adhesin. As far as we know, this is the first non-pathogenic monoxenic strain reported. Clone A (strain HM1:IMSS), a highly virulent clone with pathogenic zymodeme type II, and which has the 112-kDa adhesin, was used as a control. Protein patterns from both clones were almost identical in one-dimensional gels. In two-dimensional gels, differences in high-molecular-weight proteins were detected. Clone MAV-I adhered and phagocytosed only 12% of the red blood cells adhered and phagocytosed by clone A. MAV-I trophozoites did not destroy cell culture monolayers and did not produce hepatic abscesses in hamsters. They also showed deficiency in protease activity. The absence of virulence in clone MAV-I correlated directly with the absence of a functional 112-kDa adhesin, supporting the role that this protein plays in virulence. © 1990.
Filiaciones:
Vargas M.A.:
Departamento de Genética y Biología Molecular, CINVESTAV IPN, Mexico City, Mexico
Isibasi A.:
Laboratorio de Inmunoquímica, Unidad de Investigación Biomédica IMSS, Mexico City, Mexico
Kumate J.:
Secretaría de Salud Pública, Mexico City, Mexico
Orozco E.:
Departamento de Genética y Biología Molecular, CINVESTAV IPN, Mexico City, Mexico
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