Sperm quality in naturally infected rams with Brucella ovis
Por:
Maria Carrera-Chavez, Jose, Quezada-Casasola, Andres, Perez-Eguia, Eduardo, Fabian Itza-Ortiz, Mateo, Luis Gutierrez-Hernandez, Jose, Alberto Quintero-Elisea, Juan, Luis Tortora-Perez, Jorge
Publicada:
1 nov 2016
Resumen:
Ovine epididymitis caused by Brucella ovis is a chronic disease,
characterized by testicular and epididymal alterations, which cause loss
of fertility in rams. However, infected rams without clinical signs, may
have decreased fertility and be excreting the bacteria. The aim of this
study was to evaluate sperm quality in serologically positive to B. ovis
naturally infected rams. Seminal samples were obtained of 207 rams to
evaluate seminal volume, sperm concentration, mass motility, vitality,
presence of somatic cells and percentage of abnormalities. Clinical
examination was performed by palpation. The serological response against
B. ovis was evaluated by double immunodiffusion in gel. The percentage
variables were analyzed by GENMOD procedure and numerical variables were
analyzed by ANOVA and differences were assessed using Tukey's test.
18.84% of the rams were positive against B. ovis. There was no
difference in scrotal circumference and volume (P > 0.05) between
positive and negative animals for B. ovis. Sperm concentration, mass
motility, vitality, and the percentage of sperm with abnormalities, were
modified in positive animals (P < 0.05), regardless of the presence of
palpable lesions (P < 0.05). The percentage of rams with lesions in the
epididymis and severe presence of somatic cells was higher in positive
animals (P < 0.05). B. ovis infection adversely affects semen
characteristics, due to an increase in epididymal lesions and by the
severe presence of inflammatory cells in the ejaculate. Serological
analysis to determine possible infection by B. ovis, should be
considered as a component of the evaluation of fertility in rams,
considering that sperm quality was affected in rams without lesions, but
seropositive for B. ovis. (C) 2016 Elsevier B.V. All rights reserved.
Filiaciones:
Maria Carrera-Chavez, Jose:
Univ Autonoma Ciudad Juarez, Inst Ciencias Biomed, Dept Ciencias Vet, Anillo Envolvente Pronaf & Estocolmo S-N, Ciudad Juarez 35315, Chihuahua, Mexico
Quezada-Casasola, Andres:
Univ Autonoma Ciudad Juarez, Inst Ciencias Biomed, Dept Ciencias Vet, Anillo Envolvente Pronaf & Estocolmo S-N, Ciudad Juarez 35315, Chihuahua, Mexico
Perez-Eguia, Eduardo:
Univ Autonoma Ciudad Juarez, Inst Ciencias Biomed, Dept Ciencias Vet, Anillo Envolvente Pronaf & Estocolmo S-N, Ciudad Juarez 35315, Chihuahua, Mexico
Fabian Itza-Ortiz, Mateo:
Univ Autonoma Ciudad Juarez, Inst Ciencias Biomed, Dept Ciencias Vet, Anillo Envolvente Pronaf & Estocolmo S-N, Ciudad Juarez 35315, Chihuahua, Mexico
Luis Gutierrez-Hernandez, Jose:
Inst Nacl Invest Forestales Agr & Forestales, Ctr Nacl Invest Disciplinaria Microbiol CENID Mic, Km 15-5 Carretera Fed Mexico Toluca, Mexico City, DF, Mexico
Alberto Quintero-Elisea, Juan:
Univ Autonoma Ciudad Juarez, Inst Ciencias Biomed, Dept Ciencias Vet, Anillo Envolvente Pronaf & Estocolmo S-N, Ciudad Juarez 35315, Chihuahua, Mexico
Luis Tortora-Perez, Jorge:
Univ Nacl Autonoma Mexico, Fac Estudios Super Cuautitlan, Km 2-5 Carretera Cuautitlan Teoloyucan, Cuautitlan 54714, Estado De Mexic, Mexico
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