High Virulence and Antifungal Resistance in Clinical Strains of Candida albicans
Por:
Monroy-Perez, Eric, Luz Paniagua-Contreras, Gloria, Rodriguez-Purata, Pamela, Vaca-Paniagua, Felipe, Vazquez-Villasenor, Marco, Diaz-Velasquez, Clara, Uribe-Garcia, Alina, Vaca, Sergio
Publicada:
1 ene 2016
Resumen:
Antifungal resistance and virulence properties of Candida albicans are a
growing health problem worldwide. To study the expression of virulence
and azole resistance genes in 39 clinical strains of C. albicans, we
used a model of infection of human vaginal epithelial cells with C.
albicans strains isolated from Mexican women with vulvovaginal
candidiasis (VVC). The strains were identified by PCR amplification of
the ITS1 and ITS2 regions of rRNA. The detection and expression of
virulence genes and azole resistance genes MDR1 and CDR1 were performed
using PCR and RT-PCR, respectively. All strains were sensitive to
nystatin and 38 (97.4%) and 37 (94.9%) were resistant to ketoconazole
and fluconazole, respectively. ALS1, SAP4-SAP6, LIP1, LIP2, LIP4, LIP6,
LIP7, LIP9, LIP10, and PLB1-PLB2 were present in all strains; SAP1 was
identified in 37 (94.8%) isolates, HWP1 in 35 (89.7%), ALS3 in 14
(35.8%), and CDR1 in 26 (66.6%). In nearly all of the strains, ALS1,
HWP1, SAP4-SAP6, LIP1-LIP10, PLB1, and PLB2 were expressed, whereas CDR1
was expressed in 20 (51.3%) and ALS3 in 14 (35.8%). In our in vitro
model of infection with C. albicans, the clinical strains showed
different expression profiles of virulence genes in association with the
azole resistance gene CDR1. The results indicate that the strains that
infect Mexican patients suffering from VVC are highly virulent and
virtually all are insensitive to azoles.
Filiaciones:
Monroy-Perez, Eric:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Ave Barrios 1, Tlalnepantla 54090, Mex, Mexico
Luz Paniagua-Contreras, Gloria:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Ave Barrios 1, Tlalnepantla 54090, Mex, Mexico
Rodriguez-Purata, Pamela:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Ave Barrios 1, Tlalnepantla 54090, Mex, Mexico
Vaca-Paniagua, Felipe:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Ave Barrios 1, Tlalnepantla 54090, Mex, Mexico
Vazquez-Villasenor, Marco:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Ave Barrios 1, Tlalnepantla 54090, Mex, Mexico
Diaz-Velasquez, Clara:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Ave Barrios 1, Tlalnepantla 54090, Mex, Mexico
Uribe-Garcia, Alina:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Ave Barrios 1, Tlalnepantla 54090, Mex, Mexico
Vaca, Sergio:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Ave Barrios 1, Tlalnepantla 54090, Mex, Mexico
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