A non-hepatotropic parasite infection increases mortality in the acetaminophen-induced acute liver failure murine model: possible roles for IL-5 and IL-6
Por:
de Leon-Nava, Marco A., Alvarez-Delgado, Carolina, Donis-Maturano, Luis, Hernandez-Ruiz, Joselin, Manjarrez-Reyna, Aaron N., Cruz-Aviles, Edgar, Leon-Cabrera, Sonia, Morales-Montor, Jorge, Fragoso, Jose M., Escobedo, Galileo
Publicada:
1 dic 2016
Resumen:
We evaluated the effects of a non-hepatotropic parasite infection
(Taenia crassiceps) on the outcome of acetaminophen-induced acute liver
failure in mice. Uninfected and T. crassiceps infected mice orally
received either 300 mg/kg acetaminophen or water as vehicle (n = 5 per
group). Survival analysis, hepatocyte necrosis, alanine aminotransferase
(ALT) levels, CYP2E1 protein, interleukin (IL-) 5, and IL-6 were
assessed for all groups. All infected mice died within 16 h after
exposure to acetaminophen (Tc+APAP group), whereas only one-third of
uninfected animals exposed to acetaminophen (APAP group) died.
Uninfected (Control group) and infected (Tc group) mice that received
the vehicle showed no liver damage. Tc+APAP mice exhibited massive liver
necrosis characterised by marked balloning degeneration of hepatocytes
and higher serum ALT compared to Control, Tc, and APAP animals. Liver
tissue from Tc+APAP mice also displayed increased expression of CYP2E1
protein and higher mRNA and protein levels of IL-5 and IL-6 compared to
the other groups. These findings suggest that non-hepatotropic parasite
infections may increase mortality following acute liver failure by
promoting hepatocyte necrosis via IL-5 and IL-6-dependent CYP2E1
overproduction. This study identifies new potential risk factors
associated with severe acute liver failure in patients.
Filiaciones:
de Leon-Nava, Marco A.:
Ctr Invest Cient & Educ Super Ensenada, Dept Innovac Biomed, Ensenada, Baja California, Mexico
Alvarez-Delgado, Carolina:
Ctr Invest Cient & Educ Super Ensenada, Dept Innovac Biomed, Ensenada, Baja California, Mexico
Donis-Maturano, Luis:
Ctr Invest Cient & Educ Super Ensenada, Dept Innovac Biomed, Ensenada, Baja California, Mexico
Hernandez-Ruiz, Joselin:
Univ Nacl Autonoma Mexico, Hosp Gen Mexico Dr Eduardo Liceaga, Unidad Invest Med Expt, Fac Med,Lab Higado Pancreas & Motilidad, Mexico City, DF, Mexico
Manjarrez-Reyna, Aaron N.:
Univ Nacl Autonoma Mexico, Hosp Gen Mexico Dr Eduardo Liceaga, Unidad Invest Med Expt, Fac Med,Lab Higado Pancreas & Motilidad, Mexico City, DF, Mexico
Cruz-Aviles, Edgar:
Univ Nacl Autonoma Mexico, Hosp Gen Mexico Dr Eduardo Liceaga, Unidad Invest Med Expt, Fac Med,Lab Higado Pancreas & Motilidad, Mexico City, DF, Mexico
Leon-Cabrera, Sonia:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Carrera Med Cirujano, Los Reyes Iztacala, Mexico
Morales-Montor, Jorge:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Inmunol, Mexico City, DF, Mexico
Fragoso, Jose M.:
Inst Nacl Cardiol Ignacio Chavez, Dept Biol Mol, Mexico City, DF, Mexico
Escobedo, Galileo:
Univ Nacl Autonoma Mexico, Hosp Gen Mexico Dr Eduardo Liceaga, Unidad Invest Med Expt, Fac Med,Lab Higado Pancreas & Motilidad, Mexico City, DF, Mexico
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