Effect of Cross-Sex Hormonal Replacement on Antioxidant Enzymes in Rat Retroperitoneal Fat Adipocytes
Por:
Perez-Torres, Israel, Guarner-Lans, Veronica, Zuniga-Munoz, Alejandra, Velazquez Espejel, Rodrigo, Cabrera-Orefice, Alfredo, Uribe-Carvajal, Salvador, Pavon, Natalia
Publicada:
1 ene 2016
Resumen:
We report the effect of cross-sex hormonal replacement on antioxidant
enzymes from rat retroperitoneal fat adipocytes. Eight rats of each
gender were assigned to each of the following groups: control groups
were intact female or male (F and M, resp.). Experimental groups were
ovariectomized F (OvxF), castrated M (CasM), OvxF plus testosterone
(OvxF + T), and CasM plus estradiol (CasM + E-2) groups. After
sacrifice, retroperitoneal fat was dissected and processed for
histology. Adipocytes were isolated and the following enzymatic
activities were determined: Cu-Zn superoxide dismutase (SOD), catalase
(CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST),
and glutathione reductase (GR). Also, glutathione (GSH) and lipid
peroxidation (LPO) were measured. In OvxF, retroperitoneal fat increased
and adipocytes were enlarged, while in CasM rats a decrease in
retroperitoneal fat and small adipocytes are observed. The cross-sex
hormonal replacement in F rats was associated with larger adipocytes and
a further decreased activity of Cu-Zn SOD, CAT, GPx, GST, GR, and GSH,
in addition to an increase in LPO. CasM + E-2 exhibited the opposite
effects showing further activation antioxidant enzymes and decreases in
LPO. In conclusion, E-2 deficiency favors an increase in retroperitoneal
fat and large adipocytes. Cross-sex hormonal replacement in F rats
aggravates the condition by inhibiting antioxidant enzymes.
Filiaciones:
Inst Nacl Cardiol Ignacio Chavez, Dept Pathol, Juan Badiano 1,Secci 16, Mexico City 14080, DF, Mexico
Inst Nacl Cardiol Ignacio Chavez, Dept Physiol, Juan Badiano 1,Secci 16, Mexico City 14080, DF, Mexico
Univ Nacl Autonoma Mexico, Dept Mol Genet, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Inst Nacl Cardiol Ignacio Chavez, Dept Pharmacol, Juan Badiano 1,Secci 16, Mexico City 14080, DF, Mexico
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