Role of porcine serum haptoglobin in the host-parasite relationship of Taenia solium cysticercosis
Por:
Navarrete-Perea, Jose, Toledano-Magana, Yanis, De la Torre, Patricia, Sciutto, Edda, Jose Bobes, Raul, Soberon, Xavier, Pedro Laclette, Juan
Publicada:
1 jun 2016
Resumen:
Human and porcine cysticercosis is a parasitic disease caused by the
larval stage (cysts) of the tapeworm Taenia solium. Cysts may live in
several host tissues such as skeletal muscle or brain. We have
previously described the presence of host haptoglobin (Hp) and
hemoglobin (Hb) in different protein extracts of the T. solium cysts.
Here, we report the binding of host Hp and Hb to a number of cyst
proteins, evaluated through measuring electrophoretic and light
absorbance changes.
In the sera obtained from 18 cysticercotic pigs, Hp-Hb complexes were
abundant, whereas free Hp was undetectable. In contrast, in the sera
from non 18 cysticercotic pigs, Hp-Hb and free Hp were found. In the
soluble protein fraction of cysts tissue, free Hp was detected showing a
considerable Hb-binding ability, whereas in the vesicular fluid, Hp is
mainly bound to Hb. Interestingly, assays carried out with the insoluble
fraction of T. solium cysts tissue, showed binding of Hp and Hp-Hb in a
saturable way, suggesting the existence of specific interactions. Our
results suggested that the parasite can take advantage of the uptaken
host Hp and Hb, either free or in complexes, as a source of iron or as a
way to modulate the inflammatory response surrounding the T. solium
cysts. (C) 2016 Elsevier B.V. All rights reserved.
Filiaciones:
Navarrete-Perea, Jose:
Univ Nacl Autonoma Mexico, Inst Biomed Res, Dept Immunol, Mexico City 04510, DF, Mexico
Inst Nacl Med Genom, Perifer Sur 4809, Mexico City 14610, DF, Mexico
Toledano-Magana, Yanis:
Univ Nacl Autonoma Mexico, Inst Quim, Mexico City 04510, DF, Mexico
De la Torre, Patricia:
Univ Nacl Autonoma Mexico, Inst Biomed Res, Dept Immunol, Mexico City 04510, DF, Mexico
Sciutto, Edda:
Univ Nacl Autonoma Mexico, Inst Biomed Res, Dept Immunol, Mexico City 04510, DF, Mexico
Jose Bobes, Raul:
Univ Nacl Autonoma Mexico, Inst Biomed Res, Dept Immunol, Mexico City 04510, DF, Mexico
Soberon, Xavier:
Inst Nacl Med Genom, Perifer Sur 4809, Mexico City 14610, DF, Mexico
Univ Nacl Autonoma Mexico, Inst Biotecnol, Ave Univ 2001, Cuernavaca 62210, Morelos, Mexico
Pedro Laclette, Juan:
Univ Nacl Autonoma Mexico, Inst Biomed Res, Dept Immunol, Mexico City 04510, DF, Mexico
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