PHYLOGENETIC ANALYSIS USING THE 28S rRNA GENE REVEALS THAT THE GENUS PARACREPTOTREMA (DIGENEA: ALLOCREADIIDAE) IS NOT MONOPHYLETIC; DESCRIPTION OF TWO NEW GENERA AND ONE NEW SPECIES
Por:
Perez-Ponce de Leon, Gerardo, Pinacho-Pinacho, Carlos D., Mendoza-Garfias, Berenit, Choudhury, Anindo, Garcia-Varela, Martin
Publicada:
1 feb 2016
Resumen:
This study investigates the systematics of Paracreptotrema Choudhury,
Perez-Ponce de Leon, Brooks and Daverdin, 2006 using morphological data
(stained whole mounts and scanning electron microscopy) and partial
sequences of the 28S ribosomal rRNA gene, obtained from freshly
collected material. In total, 484 specimens representing 4 species,
i.e., Paracreptotrema blancoi (157), Paracreptotrema profundulusi (12),
Paracreptotrema rosenthali (8), and Paracreptotrema blancoi sensu
Salgado-Maldonado et al. (2011) (307) were collected. Existing museum
depositions were also studied. The 28S rRNA gene sequences of these
Paracreptotrema spp. were aligned, along with sequences from 22 other
allocreadiids and 4 other non-allocreadiid xiphidiatan species. Bayesian
inference and maximum likelihood analyses indicated a paraphyletic
Paracreptotrema split into 3 clades: 1 comprising P. blancoi and P.
rosenthali that was sister to a clade formed by 3 other species of
allocreadiids (species of Wallinia, Creptotrematina, and Auriculostoma)
typically found in characid fishes, a second clade formed solely by
Paracreptotrema heterandriae as the sister taxon of the aforementioned
species, and a third by P. profundulusi and specimens erroneously
identified as P. blancoi. Two new taxa were erected to reflect these
results: Paracreptotrematoides for Paracreptotrema heterandriae, and
Pseudoparacreptotrema for Paracreptotrema profundulusi and P.
macroacetabulata (the species erroneously identified as P. blancoi from
profundulids across Middle America). Closer consideration of the
morphology corroborates these findings. The revised systematics also
indicated that Paracreptotrema spp. are found in poeciliids, whereas
Pseudoparacreptotrema spp. parasitize profundulids. The study
demonstrates the value of an integrative taxonomy approach to address
the apparently complicated systematics of the allocreadiids.
Filiaciones:
Perez-Ponce de Leon, Gerardo:
Univ Nacl Autonoma Mexico, Inst Biol, Ap Postal 70-153, Mexico City 04510, DF, Mexico
Pinacho-Pinacho, Carlos D.:
Univ Nacl Autonoma Mexico, Inst Biol, Ap Postal 70-153, Mexico City 04510, DF, Mexico
Mendoza-Garfias, Berenit:
Univ Nacl Autonoma Mexico, Inst Biol, Ap Postal 70-153, Mexico City 04510, DF, Mexico
Choudhury, Anindo:
Univ Nacl Autonoma Mexico, Inst Biol, Ap Postal 70-153, Mexico City 04510, DF, Mexico
St Norbert Coll, Div Nat Sci, 100 Grant St, De Pere, WI 54115 USA
Garcia-Varela, Martin:
Univ Nacl Autonoma Mexico, Inst Biol, Ap Postal 70-153, Mexico City 04510, DF, Mexico
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